คณะวิทยาศาสตร์
Listeriosis is a severe foodborne illness characterized by a fatality rate exceeding 30%, attributed to the pathogen Listeria monocytogenes. This study evaluated 160 lactic acid bacteria (LAB) isolated from Thai pickled crabs for their potential as agents against L. monocytogenes and for their probiotic properties and probiogenomic characteristics. Among these strains, strain DRC3-2 exhibited activity through the synthesis of bacteriocin DRC3-2, which significantly inhibited L. monocytogenes ATCC 19115 in spot-on-lawn assays. Phenotypic and whole-genome analyses revealed that strain DRC3-2 thrived in environments with 2-6% NaCl, pH values ranging from 3 to 9, and temperatures between 25 and 45°C. Based on average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) values, strain DRC3-2 was taxonomically classified as Lactococcus lactis subsp. hordinae. The production of bacteriocin DRC3-2 peaked during the late stationary phase, following its synthesis in the early exponential growth phase. BAGEL4 analysis identified the putative novel bacteriocin DRC3-2 as lactococcin A and B, with respective bit-scores of 40.05 and 36.58. In silico safety assessments confirmed the nonpathogenic nature of strain DRC3-2 in humans, highlighting its absence of antibiotic resistance genes. Finally, this investigation underscores the novel bacteriocin DRC3-2 for application in the prevention and treatment of L. monocytogenes infections.
คณะอุตสาหกรรมอาหาร
Spent coffee grounds (SCGs) are waste from coffee drink process, which are rich of a varieties of nutrients. This research applied SCGs as ingredient in cracker. The optimized formula and process are studied as well as addition of different levels of SCGs were studied. It was found that addition of SCGs in cracker had hedonic score in high level from panels, especially panels who drink coffee. Moreover, it was observed that SCGs could increase nutrients especially carbohydrate and fiber to the product.
คณะอุตสาหกรรมอาหาร
In the development of high protein jasmine rice products, hydrocolloids, HPMC at 0, 0.25, 0.5 and 1% w/v and MD at 10% w/v were used. This hydrocolloid contained 30% w/v dissolved protein and was coated with raw jasmine rice. It was found that different amounts of HPMC affected the adhesion of proteins in rice. Then, the hydrocolloid with the best adhesion, 0.25% w/v, was used to find the optimum amount for coating rice at ratios of 1:3 and 1:5, which affected protein content, texture, color, water retention and sensory acceptability.
คณะอุตสาหกรรมอาหาร
This research aims to develop plant-based Thai traditional sausage(Sai oua) using young jackfruit as the main raw material. To be an alternative to replace meat by studying the development of formula, changes in physical and physical chemistry during the preservation of the product, young jackfruit has dominant properties of meat-like fibres and can absorb the smell and taste of spices well. The results of the study found that young jackfruit that have been boiled at 100 degrees Celsius for 30 minutes have the fibre closest to cooked chicken. In addition, the study of changes during storage at different temperatures found that the colour and Water Activity (Aw) have changed slightly. While the pH value (pH) decreased and the value of Thiobarbituric Acid Reactive Substances (T-BARS) increased. In terms of texture, it was found that the toughness increased and the elasticity decreased. Compared to the control formula, this study suggests that young jackfruit is a suitable raw material for the production of fillings from plants.
คณะอุตสาหกรรมอาหาร
This research focuses on the development of mango powder using the foam-mat drying method, which is an effective technique for preserving the quality of fruit and vegetable products. Hydroxypropyl Methylcellulose (HPMC) was used as a foaming agent. The study evaluated the effects of HPMC on the chemical and physical properties, antioxidant activity, and shelf life of mango powder. The findings indicated that HPMC plays a crucial role in improving the foam stability before drying and enhancing the quality of the dried powder. This research provides a valuable approach to adding value to substandard mango yields and reducing agricultural waste. It also contributes to the development of high-nutritional processed food products with extended shelf life.
คณะอุตสาหกรรมอาหาร
Plant-based refers to food or products that are primarily made from plants. It can be divided into two categories: one is food that comes entirely from plants and does not include any animal products, and the other is food that contains small amounts of animal products, such as products that contain milk and eggs in limited quantities, which may also be considered part of the definition of plant-based. Plant-based meat products that closely resemble real meat and attract consumers are considered a relatively new innovation. Although tofu, tempeh, and seitan have been around for a long time, recent discoveries have led to the production of plant-based meat products that provide a sensory experience, making it difficult for consumers to distinguish between real meat and plant-based meat. Furthermore, the development of plant-based food products must prioritize quality and safety to maximize consumer benefits. Textured Vegetable Protein (TVP) is a plant-based protein made from soybeans using an extruder. It is used as a primary ingredient in the production of plant-based food products due to several advantages. These include: • High Protein Content: TVP is made from soybeans with the fat extracted, resulting in a high protein content. • Texture: When rehydrated, TVP has a texture that closely resembles meat. • Versatility: TVP has a neutral flavor, allowing it to easily absorb the flavors of various seasonings and sauces. • Cost-Effectiveness: Compared to other protein sources, TVP is relatively inexpensive while providing desirable characteristics. These benefits make TVP an attractive option in the production of plant-based foods. This study focuses on developing TVP into a plant-based crab cake and investigating the shelf life of the product in a tightly sealed container under refrigeration. It also analyzes the hygiene and cleanliness of the production process and how these factors affect the presence or growth of microorganisms that may pose a risk to consumers, referencing the cold food safety standards of Thailand. Finally, recommendations for cleaning operational areas will be provided to establishments as a guideline for developing preliminary food safety procedures in laboratory settings.
คณะศิลปศาสตร์
As a value-added product of locally sourced fruits in Phang Nga, stevia-sweetened jelly offers a healthy and sustainable option for consumers seeking reduced sugar intake. This product has the potential to become a popular souvenir, promoting local agriculture and boosting the regional economy.
คณะอุตสาหกรรมอาหาร
The reuse of cooking oil in food preparation leads to oil degradation and the formation of harmful compounds due to oxidation. This study focuses on enhancing the stability of used palm oil through ultrasound-assisted infusion with three varieties of banana blossoms: Kluai Khai, Kluai Hom, and Kluai Nam Wa, which are rich in phenolic compounds and antioxidants. The research investigates the restoration of used palm oil by infusing dried and powdered banana blossoms using ultrasonic treatment at different temperatures and durations. The quality of the infused oil was evaluated through physical (water activity, moisture content, and color), chemical (peroxide value, acid value, and Thio barbituric acid reactive substances), and antioxidant activity (DPPH, ABTS, and FRAP) analyses.
คณะวิทยาศาสตร์
With the urgent need for rapid screening of Aflatoxin B1 (AFB1) due to its association with increased liver cirrhosis and hepatocellular carcinoma cases from contaminated agricultural foods, we propose a novel electrochemical aptasensor. This aptasensor is based on trimetallic nanoparticles AuPt-Ru supported by reduced graphene oxide (AuPt-Ru/RGO) modified on a low-cost and disposable goldleaf electrode (GLEAuPt-Ru/RGO) for detection of AFB1. The trimetallic nanoparticle AuPt-Ru was synthesized using an ultrasonic-driven chemical reduction method. The synthesized AuPt-Ru exhibited a waxberry-like appearance, with AuPt core-shell structure and ruthenium dispersed over the particles. The average particle size was 57.35 ± 8.24 nm. The AuPt-Ru was integrated into RGO sheets (inner diameter of 0.5 to 1.6 µm) in order to enhance electron transfer efficiency and increase the specific immobilizing surface area of the thiol-5’-terminated modified aptamer (Apt) to target AFB1. With a large electrochemical surface area and low electrochemical impedance, GLEAuPt-Ru/RGO displays ultra-high sensitivity for AFB1 detection. Differential pulse voltammetry (DPV) measurements revealed a linear range for AFB1 detection range from 0.3 to 30.0 pg mL-1 (R2 = 0.9972), with a limit of detection (LOD, S/N = 3) and a limit of quantification (LOQ, S/N = 10) of 0.009 pg mL-1 and 0.031 pg mL-1, respectively. The developed aptasensor also demonstrated excellent accuracy in real agricultural products, including dried red chili, garlic, peanut, pepper, and Thai jasmine rice, achieving recovery rates between 94.6 and 107.9%. The fabricated aptamer-based GLEAuPt-Ru/RGO performance is comparable to that of a modified commercial electrode, which has great potential application prospects for detecting AFB1 in agricultural products.