Listeriosis is a severe foodborne illness characterized by a fatality rate exceeding 30%, attributed to the pathogen Listeria monocytogenes. This study evaluated 160 lactic acid bacteria (LAB) isolated from Thai pickled crabs for their potential as agents against L. monocytogenes and for their probiotic properties and probiogenomic characteristics. Among these strains, strain DRC3-2 exhibited activity through the synthesis of bacteriocin DRC3-2, which significantly inhibited L. monocytogenes ATCC 19115 in spot-on-lawn assays. Phenotypic and whole-genome analyses revealed that strain DRC3-2 thrived in environments with 2-6% NaCl, pH values ranging from 3 to 9, and temperatures between 25 and 45°C. Based on average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) values, strain DRC3-2 was taxonomically classified as Lactococcus lactis subsp. hordinae. The production of bacteriocin DRC3-2 peaked during the late stationary phase, following its synthesis in the early exponential growth phase. BAGEL4 analysis identified the putative novel bacteriocin DRC3-2 as lactococcin A and B, with respective bit-scores of 40.05 and 36.58. In silico safety assessments confirmed the nonpathogenic nature of strain DRC3-2 in humans, highlighting its absence of antibiotic resistance genes. Finally, this investigation underscores the novel bacteriocin DRC3-2 for application in the prevention and treatment of L. monocytogenes infections.
Listeria monocytogenes เป็นเชื้อก่อโรคที่มักพบในอาหารที่มีการปนเปื้อน สามารถอยู่รอดในสภาวะที่รุนแรง เช่น อุณหภูมิต่ำ ความเป็นกรดสูง ความเข้มข้นของเกลือสูง ซึ่งทำให้มีความเสี่ยงอย่างมากในระบบย่อยอาหารของมนุษย์ ดังนั้นจึงศึกษาแบคทีเรียกรดแลคติกที่มีความสำคัญในอุตสาหกรรมอาหารและยาเนื่องจากมีคุณสมบัติเป็นโพรไบโอติก และแบคทีเรียกรดแลคติกบางชนิดที่สามารถผลิตแบคเทอริโอซินหรือเปปไทด์ต้านจุลชีพที่มีฤทธิ์ทางชีวภาพ และแบคเทอริโอซินที่มีประสิทธิภาพในการยับยั้งแบคทีเรียก่อโรค ซึ่งถือว่าเป็นทางเลือกที่น่าสนใจแทนการใช้ยาปฎิชีวนะแบบดั้งเดิม เนื่องจากช่วยลดการพัฒนาการดื้อยาของแบคทีเรีย นอกจากนี้แบคเทอริโอซินยังมีความเสถียรสูง สามารถทนต่ออุณหภูมิที่รุนแรงและสภาวะเป็นกรดได้ อีกทั้งยังไม่เป็นพิษต่อเซลล์มนุษย์ การศึกษานี้จึงมุ่งหมายเพื่อศึกษาแบคเทอริโอซินชนิดใหม่จาก Lactococcus lactis subsp. hordinae DCR3-2 ซึ่งแยกได้จากปูดอง เพื่อประเมินฤทธิ์การต้านจุลชีพของแบคทีเรียชนิดนี้ และการวิเคราะห์ทางbioinformatics เพื่อยืนยันว่าสายพันธุ์ DCR3-2 มีความปลอดภัยสำหรับมนุษย์และมีคุณสมบัติโพรไบโอติก
คณะวิทยาศาสตร์
This work presents the fabrication of the handheld meter for potentiometric detection of Hg (II). The meter was constructed based on using an ion-sensitive field-effect transistor (ISFET) platform. The developed meter provides high accuracy and precision (%Recovery was in the range of 92.55 - 109.32 and %RSD was 2.38). It was applied to the analysis of cosmetic samples. The results by the developed electrode were not significantly different at a 95% confidence level compared to the results by using ICP-OES.
คณะครุศาสตร์อุตสาหกรรมและเทคโนโลยี
Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang has a vision for sustainable excellence. The mission is to develop learners to be ready for the digital world, develop educational innovations using research as a base, strategic management with good governance, and academic services that benefit society. In this activity, the group of students joined with the Embassy of the Russian Federation in the Kingdom of Thailand, the working group of the Thai Silk and Culture Promotion Association, and the working group of the National Research Office (NRCT) to integrate knowledge to design a silk outfit that combines Thai and Russian cultures, create a network of cooperation in arts, culture, technology, innovation, and dissemination of knowledge and the beauty of Thai silk. The objective is to develop the potential of teachers and students in creative design, listen to the work guidelines from the working group of the Thai Silk and Culture Promotion Association, the working group of the National Research Office (NRCT) via an online meeting. The team of teachers and students from King Mongkut's Institute of Technology Ladkrabang under the name of the "Love Silk" group designed a Thai silk outfit that combines cultures with the identity of Thai silk and studying traditions and cultures of the Russian Federation's clothing. They studied related literature and research documents and integrated knowledge into the design process, inspired by the concept of the Rajapataen outfit. Since the reign of King Chulalongkorn (Rama V) in 1872, together with the clothing culture of the Russian Federation, emphasizing Thai silk, this concept has gone through the process of creating and selecting the design concept (Concept Generation and Selection). The concept received from the embassy was first submitted for feedback on July 25, 2024. There was a suggestion to add more uniqueness to Thai silk through a fashion show presentation by the wife and grandson of the Ambassador of the Russian Federation. Therefore, the designed outfits are 1 set of women's clothes and 1 set of boys' clothes. The women's set has an inner shirt adapted from the royal outfit using silk fabric, Kon Ka-ed pattern, with two separate pieces: 1 shirt and 1 skirt. The jacket is a modified long suit style, plain silk, dark pink and red. The boys' set has a long-sleeved shirt adapted from the contemporary royal style, tailored with cream silk, long slacks tailored with raw betel silk, and a collarless coat with blue silk and lotus pattern. Adapted from the Rajapattan suit with a long collar in an international style. On August 2, 2024, the designed suit and the prototype of the raw fabric suit were brought to the ambassador's wife and nephew to try on. On August 30, 2024, the ambassador was met for the 4th time to bring the silk suit that was cut into a real silk suit. The shirt was given to the ambassador's wife and nephew to try on. It was worn to join the fashion show in the 13th "Thai Silk to the World" Silk Festival at the Naval Auditorium, where lecturers and students from the Faculty of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang, joined the fashion show in the finale round, which proceeded smoothly. After the event, the working group of the Thai Silk and Culture Promotion Association brought the clothes designed and tailored by the "Rak Prae Mai" team to exhibit Thai Silk to the World Exhibition from September 1-8, 2024 at the Emsphere Shopping Mall. The team summarized the report and compiled a complete report. In the implementation of this project, the Faculty of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang has received support and facilitation throughout the project. The budget support from the National Research Council of Thailand (NRCT), the support of fabrics for sewing from the Thai Silk and Culture Promotion Association, and the information for designing valuable silk dresses from the Embassy of the Russian Federation in the Kingdom of Thailand are very important factors that made this operation a success. It is a very important experience for the team of teachers and students of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang. We sincerely hope to receive good cooperation in the future.
คณะเทคโนโลยีการเกษตร
The objective of this experiment was to determine the effect of nitrogen and potassium concentration combination with photoperiod on the growth of Viola in a plant factory to increase the quality of the products, reduce the production time and increase the production cycle throughout the year. The experimental plan was 3x3 Factorial in CRD with nine treatments and three replications (six plants per replication). The factor of this study was two factors; the first factor was three different concentrations of nitrogen and potassium in ratios of 1:1, 1:2 and 2:1. The second factor was the application of different photoperiods. There were 1) 24-hours photoperiod, 2) 8-hours light/16-hours dark photoperiod (Induced flowering state: 13-hours light/11-hours dark photoperiod) and 3) 5-hours light/3-hours dark photoperiod. Controlled temperature at 25 °C, the EC=1.5-2.0 mS/cm and the pH=5.8-6.5 in all treatment. The result showed that the concentration of N: K in the ratio of 1:1 combined with 24-hour photoperiod was the most vegetative growth and also maximizes reproductive growth. The overall great sensory evaluation was an acceptable level and suitable for cooking or decorating dishes. Therefore, the concentration of N: K in the ratio of 1:1 combined with 24-hour photoperiod is the best treatment to increase the quality of the product, reduce the production time of viola flowers in each cycle from 90-100 days down to 43-45 days which is good for farmers.