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ชิ้นงานKMITL Expo 2025Cluster 2025ป. โท
DISPOSABLE
AND
LOW-
COST
GOLDLEAF
ELECTRODE-
DECORATED
AuPt-
Ru/
RGO
NANOCOMPOSITE
FOR
ULTRASENSITIVE
ELECTROCHEMICAL
APTASENSOR
QUANTIFICATION
OF
AFLATOXIN
B1
IN
AGRICULTURAL
PRODUCTS
คณะวิทยาศาสตร์, เคมี, วิทยาศาสตรมหาบัณฑิต สาขาวิชาเคมี
AI Translated
DISPOSABLE AND LOW-COST GOLDLEAF ELECTRODE-DECORATED AuPt-Ru/RGO NANOCOMPOSITE FOR ULTRASENSITIVE ELECTROCHEMICAL APTASENSOR QUANTIFICATION OF AFLATOXIN B1 IN AGRICULTURAL PRODUCTS

Innovation Owner

CP

Mr. CHAKKAPHAN PHO-NGERNNGAM

Student

Details

This research proposes a novel, low-cost, and disposable electrochemical aptasensor using goldleaf electrodes modified with AuPt-Ru/RGO nanocomposites for the ultrasensitive detection of Aflatoxin B1 (AFB1) in agricultural products.

This research proposes a novel electrochemical aptasensor based on trimetallic nanoparticles AuPt-Ru supported by reduced graphene oxide (AuPt-Ru/RGO) modified on a low-cost and disposable goldleaf electrode (GLEAuPt-Ru/RGO) for the detection of AFB1.

  • Nanocomposite Synthesis: Synthesized via an ultrasonic-driven chemical reduction method, exhibiting a waxberry-like appearance with an average particle size of 57.35 ± 8.24 nm.
  • Performance: The integration into RGO sheets enhances electron transfer efficiency and increases the specific immobilizing surface area for the aptamer.
  • Analytical Results: Differential pulse voltammetry (DPV) revealed a linear range of 0.3 to 30.0 pg mL-1 (R2 = 0.9972), with an LOD of 0.009 pg mL-1.
  • Real-world Application: Demonstrated excellent accuracy in samples including dried red chili, garlic, peanut, pepper, and Thai jasmine rice, with recovery rates between 94.6% and 107.9%.

Objective

To develop a low-cost goldleaf electrode modified with AuPt-Ru/RGO nanocomposites for the sensitive detection of AFB1 in agricultural products and to validate its performance against standard methods.

  1. To construct and develop goldleaf electrodes for the detection of the carcinogen AFB1.
  2. To synthesize, analyze, and study the feasibility of using trimetallic nanoparticles combined with reduced graphene oxide and aptamers to enhance specificity and improve the surface of goldleaf electrodes for AFB1 detection.
  3. To study the efficiency and evaluate the capability of GLEAuPt-Ru/RGO in detecting AFB1 in agricultural products such as dried chili, garlic, peanuts, pepper, and Thai jasmine rice.
  4. To compare the developed method with other standard detection techniques, such as HPLC-FLD.