Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Traditional preventions like brushing, while helpful, struggle to fully eradicate this resilient bacteria’s biofilm, as once it is formed, simple brushing is not enough.
คณะวิทยาศาสตร์
This study aimed to investigate the effectiveness of extracts from moringa seeds, roselle seeds, and tamarind seeds as coagulants to improve water quality in surface water sources. Extracts from these seeds serve as environmentally friendly coagulants and provide alternative options for enhancing surface water quality. The turbidity of surface water sources ranged between 14 and 24 NTU. The coagulation process used the Jar Test method, where the moringa seed, roselle seed, and tamarind seed extracts functioned as both primary coagulants and coagulant aids. In the preparation process, the seeds were finely ground and extracted using a 0.5-M sodium chloride (NaCl) solution. These extracts were then applied as coagulants to reduce turbidity and enhance water quality, with each concentration tested in 300 ml of water. The results indicated that the most effective way to remove turbidity using 2,000 mg/L of moringa seed extract, achieving a turbidity reduction of approximately 73.19% at a cost of 0.0309 baht per 300 ml of water. Followed by Tamarind seed extract, with a concentration of 4,000 mg/L, followed with a turbidity reduction of approximately 56.75% at a cost of 0.0933 baht per 300 ml. Lastly, roselle seed extract at 6,000 mg/L achieved a turbidity reduction of approximately 32.67% at a cost of 0.0567 baht per 300 ml of water.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
This work got the idea of bringing the car culture of Thai teenagers to present in a new way through our perspective. Create characters and bring various elements within the culture to combine with what we like. Whether it's stickers, posters and band shirts with acrylic paint techniques.
คณะเทคโนโลยีการเกษตร
This study aimed to evaluate the optimal edible coating formulation for 'Namdokmai Sithong' mangoes by incorporating 10% gum arabic (GA) with mangosteen peel extract (MPE) at varying concentrations (1%, 3%, and 5%), compared to a control treatment (distilled water). The coated fruits were stored at room temperature for 14 days, and their physicochemical properties were assessed. The findings indicate that the application of GA (10%) combined with MPE effectively mitigated color changes in mango flesh, suppressed disease incidence, and preserved fruit firmness. Additionally, the coating significantly delayed alterations in total soluble solids (TSS), titratable acidity (TA), vitamin C content, carotenoid levels, and phenolic compounds. Among the tested formulations, GA (10%) + MPE (1%) exhibited the highest efficacy in extending shelf life, maintaining fruit quality, and enhancing surface gloss.