Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Traditional preventions like brushing, while helpful, struggle to fully eradicate this resilient bacteria’s biofilm, as once it is formed, simple brushing is not enough.
คณะวิศวกรรมศาสตร์
Designing advanced printed circuit boards for industrial applications involves a variety of steps and methods depending on each company. From what I have learned, I have used Cadence Allegro to design printed circuit boards. This internship was designed on a variety of boards with varying levels of difficulty. Learning in this internship could not be learned in detail within the university. I had to work with many departments within Analog Devices (Thailand) Company. This design was assisted by a mentor who took care of and taught me the work, allowing me to complete the co-operative successfully.
คณะวิทยาศาสตร์
Albumin Smart Test is an innovative device for screening of kidney disease by mobile phone. The device composes of (1) container and testing device with specific reagents for the albumin detection. (2) The mobile phone, installed with "Albumin smart test" application. The test is started by dropping patient urine and the reagent. Color of the product is occurred and is captured by the application with subsequent evaluation of the albumin amount. The results is displayed on screen within 3 mins. This innovative device is simple, rapid and user-friendly.
คณะเทคโนโลยีการเกษตร
This research aims to evaluate the efficiency of nano-type oxygen diffusers at different pump power levels in sea bass nursery ponds. The study examines how varying power levels affect dissolved oxygen distribution in the water and their impact on the health, growth, and survival rates of sea bass. The findings indicate that pump power levels influence dissolved oxygen concentration, with the optimal power level improving oxygen distribution in the pond. This enhancement leads to higher survival and growth rates for sea bass. The results provide valuable insights for selecting appropriate oxygen diffusers and pump power levels in fish nursery pond systems. The experiment consisted of two conditions: 1. Without fish – This condition assessed the oxygenation capacity, oxygen transfer coefficient, oxygen transfer rate, and oxygen transfer efficiency of pumps at three different power levels. 2. With fish – This condition evaluated whether the oxygen supplied by pumps at three power levels was sufficient, based on the growth rate and survival rate of the fish in the pond. Blood counts were conducted to assess the immune response. The collected data were statistically analyzed using the RCBD method for the condition without fish and the CRD method for the condition with fish, employing SPSS software.