Innovation Owner
Mr. NATTASET CHAMNANVINIJCHAI
Student
Details
This study evaluates the synergistic antibacterial activity of Histatin-5 and Phytosphingosine against Streptococcus mutans biofilm. Results show that the combination significantly enhances antimicrobial efficacy without cytotoxicity to human gingival fibroblasts.
Dental caries is a significant global issue, with Streptococcus mutans biofilm being particularly difficult to eradicate. This study investigates the potential of Histatin-5 (HST-5) and Phytosphingosine (PHS) as therapeutic agents:
- Methods: Antimicrobial activity was assessed via time-kill assays, biofilm-forming capacity via crystal violet staining, and biofilm eradication via the TSP pin lid model. Cytotoxicity was evaluated using MTT assays on human gingival fibroblast cells (hGFs).
- Results: Both agents exhibit time- and concentration-dependent activity. Their combination demonstrates a synergistic effect, reducing IC50 values by 8-fold and 16-fold, respectively, with no observed cytotoxicity in hGFs.
- Conclusions: Combining HST-5 and PHS enhances the effectiveness of anti-infective agents against S. mutans biofilm, offering a promising approach for preventing dental caries.
Objective
This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions.
This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions.