Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Traditional preventions like brushing, while helpful, struggle to fully eradicate this resilient bacteria’s biofilm, as once it is formed, simple brushing is not enough.
คณะวิทยาศาสตร์
Direct Arc Plasma Generator with Six Nozzles, Applications of Plasma Technology and Progress in Nuclear Fusion and Thailand Tokamak-1 (TT1) Development
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
This study explores the design, production, and installation of 3D-printed modular artificial reefs (3DMARs) at Koh Khai, Chumphon Province, Thailand, through a design thinking framework. Collaborating with SCG Co., Ltd. and the Department of Marine and Coastal Resources, the research establishes design criteria and installation methods, utilizing content analysis and qualitative research. Key principles such as modularity, flexibility, environmental sustainability, and usability are identified. The user-centered approach optimizes the 3DMARs for transport and deployment, enabling local community involvement and fostering sustainable practices. The modular design supports scalability, enhancing marine habitats and coral larval settlement. Furthermore, underwater monitoring techniques enable site-specific data collection, allowing for the generation of digital twin models. This research offers a practical framework for marine ecosystem restoration and empowers coastal communities in Thailand and beyond
คณะอุตสาหกรรมอาหาร
The activities of the project's operations consist of: checking microbe on sample food, hygienic condition of cooker, containers and materials, sanitation knowledge and private sanitation and food quality of canteen and cleaning of cooker. The Food Safety Management program collaborated with the Property Management office, planned the operations, and assessed food vendors based on the SAN 20 food safety standards requirements. Using A.13 testing kits, we conducted testing for coliform bacteria contamination in food, containers, equipment, and hand contact surfaces, collecting 6 samples. These included samples such as prepared food, areas in front of the store, and food handlers' hands. Additionally, we used A.11 testing kits to test for coliform bacteria contamination in water and ice. The analysis of results, including physical, microbiological, and chemical aspects, serve as a guideline for improving the quality and safety of food production and service in the institution's canteen.