Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Traditional preventions like brushing, while helpful, struggle to fully eradicate this resilient bacteria’s biofilm, as once it is formed, simple brushing is not enough.
คณะอุตสาหกรรมอาหาร
Plant-based refers to food or products that are primarily made from plants. It can be divided into two categories: one is food that comes entirely from plants and does not include any animal products, and the other is food that contains small amounts of animal products, such as products that contain milk and eggs in limited quantities, which may also be considered part of the definition of plant-based. Plant-based meat products that closely resemble real meat and attract consumers are considered a relatively new innovation. Although tofu, tempeh, and seitan have been around for a long time, recent discoveries have led to the production of plant-based meat products that provide a sensory experience, making it difficult for consumers to distinguish between real meat and plant-based meat. Furthermore, the development of plant-based food products must prioritize quality and safety to maximize consumer benefits. Textured Vegetable Protein (TVP) is a plant-based protein made from soybeans using an extruder. It is used as a primary ingredient in the production of plant-based food products due to several advantages. These include: • High Protein Content: TVP is made from soybeans with the fat extracted, resulting in a high protein content. • Texture: When rehydrated, TVP has a texture that closely resembles meat. • Versatility: TVP has a neutral flavor, allowing it to easily absorb the flavors of various seasonings and sauces. • Cost-Effectiveness: Compared to other protein sources, TVP is relatively inexpensive while providing desirable characteristics. These benefits make TVP an attractive option in the production of plant-based foods. This study focuses on developing TVP into a plant-based crab cake and investigating the shelf life of the product in a tightly sealed container under refrigeration. It also analyzes the hygiene and cleanliness of the production process and how these factors affect the presence or growth of microorganisms that may pose a risk to consumers, referencing the cold food safety standards of Thailand. Finally, recommendations for cleaning operational areas will be provided to establishments as a guideline for developing preliminary food safety procedures in laboratory settings.
คณะวิทยาศาสตร์
This project presents the development of an automatic recycling machine for plastic bottles and cans, utilizing Machine Learning for packaging classification through image processing, integrated with smart sensor systems for quality inspection and operation control. The system connects to a Web Application for real-time monitoring and control. Once the packaging type is verified, the system automatically calculates the refund value and processes payment through e-wallet or issues cash vouchers. The system can be installed in public spaces to promote waste segregation at source, reduce contamination, and increase recycling efficiency. It also provides financial incentives to encourage public participation in waste management. This project demonstrates the potential of combining Machine Learning and smart sensor systems in developing accurate, convenient, and sustainable waste management solutions.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
The Project Urban House is an initiative focused on developing and designing urban housing solutions that address the growing demand for city living. The project emphasizes efficient space utilization, sustainability, and designs that cater to modern urban lifestyles. Key considerations include the use of eco-friendly materials, the integration of green spaces, and the implementation of smart home technologies to enhance residents' quality of life.