The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
คณะเทคโนโลยีการเกษตร
This experiment aimed to study the suitable types of polymers for coating with chlorophyll extract and the quality of cucumber seeds after coating. The experiment was planned using a Completely Randomized Design (CRD) with four replications, consisting of five methods involving seeds coated with different types of polymers: Polyvinylpyrrolidone, Sodium Alginate, Carboxy Methyl Cellulose, and Hydroxypropyl Methylcellulose, each polymer being coated alongside chlorophyll, with uncoated seeds serving as the control method. The coating substance was prepared by extracting chlorophyll from mango leaves, then mixed with each type of polymer at a concentration of 1%, using an 8% concentration of chlorophyll extract. The properties of each coating method, such as pH and viscosity of the coating substance, were examined before coating the cucumber seeds with a rotary disk coater model RRC150 at a coating rate of 1,100 milliliters per 1 kilogram of seeds. Subsequently, the seeds were dried to reach the initial moisture level using a hot air blower, and seed quality was assessed in various aspects, including seed moisture, germination rate under laboratory conditions, germination index, and seed fluorescence under a portable ultraviolet light illuminator, as well as light emission spectrum analysis using a Spectrophotometer. The experiment found that each type of polymer could be used to form a film together with chlorophyll, which had appropriate pH and viscosity for the coating without affecting seed quality and showed fluorescence on the seed surface both under portable ultraviolet light and spectral emission analysis with a Spectrophotometer. Using HPMC as the film-forming agent with chlorophyll was the most suitable method, enhancing seed fluorescence efficiency.
คณะเทคโนโลยีการเกษตร
Climate change affects agricultural systems worldwide, including Thailand, and may lead to reduced crop yields, impacting food security. Bambara groundnut is a crop with the potential to adapt to changing environments and can thrive in areas with limited resources. This research aims to study the impact of climate change on Bambara groundnut yields in Thailand using the DSSAT (Decision Support System for Agrotechnology Transfer) model, an important tool for predicting plant growth under various environmental conditions. This study utilizes climate data, soil composition, and genetic information of Bambara groundnut to simulate and analyze yield trends under future climate scenarios. Four study areas in Thailand were selected: Songkhla, Lampang, Yasothon, and Saraburi. The CSM-CROPGRO-Bambara groundnut model was used to assess the impact of changing temperature and rainfall on the growth and yield of Bambara groundnut. The results of this study are expected to provide farmers and researchers with valuable information for planning cultivation and managing peanut production in response to climate change. Additionally, the findings can help formulate policy guidelines to promote the cultivation of climate-resilient crops and support the country's food security.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
The concept for this work came from my curiosity about what would happen if, during interdimensional travel in space, a teleportation system were used. This system involves removing matter from one point and transferring it to another while maintaining its original state. If an error occurs and the matter is recreated or fused together, it could result in an experimental creature merging with the spacecraft. I choose the tardigrade as the first experimental subject for teleportation because the water bear has already been sent into space and survived. Therefore, I thought that if we were to actually test this teleportation system, the tardigrade would likely be one of the creatures chosen for experimentation.