The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
คณะครุศาสตร์อุตสาหกรรมและเทคโนโลยี
Crispy Rice-berry Snack is a product made from broken rice-berry rice that has been processed into a snack that is thin and crispy, bite-sized. Broken rice-berry rice is cooked, finely ground, and mixed with other ingredients to increase its nutritional value, such as adding plant seeds, adding plant protein nutrients, and then forming it into sheets using heat. The resulting product is a thin sheet, purple-brown in color, crispy, and has the smell of the ingredients used in the production process. It does not contain sugar or sweeteners. It is used as a snack with tea or coffee. Crispy Rice-berry Waffle is a product that contains complete nutrients, including carbohydrates, protein, and fat, which are derived from the ingredients in the production formula.
คณะอุตสาหกรรมอาหาร
This study aims to investigate the co-encapsulation technique of vitamin C and coenzyme Q10 within liposomes to enhance their stability and encapsulation efficiency and evaluate their antioxidant activity and release behavior under simulated gastrointestinal conditions. Liposomes were prepared using the High-Speed Homogenization Method, and their characteristics, including particle size, zeta potential, encapsulation efficiency, and antioxidant activity, were analyzed using DPPH, ABTS, and FRAP assays. The results demonstrated that co-encapsulation significantly improved the stability of vitamin C and coenzyme Q10 compared to single encapsulation. The liposomes exhibited high encapsulation efficiency and maintained strong antioxidant activity. The release profile under simulated gastrointestinal conditions also indicated a sustained and controlled release. These findings highlight the potential of the co-encapsulation technique in enhancing the efficacy of functional bioactive compounds, making it applicable to the food and nutraceutical industries.
คณะเทคโนโลยีสารสนเทศ
The process of treating cancer patients in the chemotherapy department at Chonburi Cancer Hospital is complicated and inconvenient due to the procedure of submitting blood test results through the personal LINE application of medical staff, which hinders workflow efficiency. Therefore, the researcher has developed a cancer patient management and tracking program in the form of a web-based application and LINE LIFF (LINE Front-end Framework) application to facilitate both medical personnel and patients. The web-based application is designed for medical personnel to monitor, schedule, and collect patient data, while the LINE application is designed for patients to submit blood test results, view appointment schedules, record symptoms after chemotherapy, log their weekly weight, and access a chatbot for consultation. This system is developed based on client-server technology, which enhances data analysis efficiency and supports automated treatment planning. As a result, the cancer treatment process becomes faster, more modern, and more efficient.