The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
คณะเทคโนโลยีสารสนเทศ
The project of game development to educate about Egyptian civilization This is intended to be a game to promote learning about ancient Egyptian civilization. The original learning may feel boring. Do not attract the attention of learners Therefore saw the presentation in the form of a game on virtual reality technology that inserts knowledge history With the aim of making learning more interesting The organizers can choose to use Unreal Engine 5.1 (Unreal Engine 5.1) and Oculus Quest II (Oculus Quest2) to develop. Within the game, players must find a way to escape from this room within the specified period of solving puzzles in various forms such as statues, traps, etc. In order to solve puzzles and leave this room, players must collect all the information inside the room. In addition, the game shows the life of the player and when the player cannot solve the puzzle correctly.
คณะวิทยาศาสตร์
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คณะวิศวกรรมศาสตร์
This project aims to propose a design for a red offal processing room in a pork processing plant that processes 500 pigs per day or 80 pigs per hour. Each pig weighs approximately 105 kilograms, with 3.47% of the weight consisting of red offal. The process involves separating liver, gall bladder, heart, lungs, spleen, and kidneys as required. These parts are then chilled in cold water to reduce their temperature to below 7°C before packaging and sealing. Sorting is based on the number of pieces and weight, depending on the type of product. The processing times of sorting chilling and packaging vary depending on the product's type and size. The design was developed using data collected from the current production line and referenced standards. The room layout was planned using Systematic Layout Planning (SLP) principles to analyze activity relationships within the room and define functional areas. Equipment sizes and the required number of operators were calculated to ensure optimal use of space. The red offal processing room was designed with an area of 56 square meters. After the layout design was completed, a 3D model was created using SketchUp 2024, and the workflow and operations were simulated and analyzed using Flexsim 2024