The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
วิทยาเขตชุมพรเขตรอุดมศักดิ์
A sweet made from flour and added to give it a chewy texture similar to chewing gum.
วิทยาลัยนวัตกรรมการผลิตขั้นสูง
The research on improving the strength of solid electrolytes aims to enhance the properties of solid electrolyte materials produced from cement and additives that help develop the cement structure to generate electricity. The main components include sodium chloride (NaCl) and graphite, which contribute to the material’s ability to generate a weak electrical current. The objective is to develop an electricity-generating flooring material. This study involves preparing a mixture of cement, water, sodium chloride (NaCl), and graphite to enhance the material’s electrical conductivity. It is highly anticipated that this research will lead to the development of concrete flooring capable of generating electricity and can be further expanded for future applications.
คณะอุตสาหกรรมอาหาร
Spent coffee grounds (SCG) are a byproduct of the coffee brewing process, and their quantity continues to increase due to the growing global coffee consumption. SCG contain beneficial compounds such as polysaccharides, dietary fibers, and antioxidants, which can be utilized in various applications, including prebiotic extraction. This study focuses on extracting prebiotics from SCG using acid hydrolysis and enzymatic hydrolysis methods to evaluate their potential in promoting the growth of beneficial gut microorganisms. The expected results of this research include adding value to coffee industry waste, reducing organic waste, and providing a sustainable approach to developing prebiotic products for use in the food and health industries. Furthermore, this study aligns with sustainable resource utilization and environmentally friendly practices.