The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.
ปัจจุบันมีงานวิจัยเกี่ยวกับโพรไบโอติกส์เพิ่มมากขึ้น เนื่องจากโพรไบโอติกส์มีประโยชน์ต่อสุขภาพมนุษย์โดยเฉพาะระบบทางเดินอาหาร เช่น ช่วยการปรับสมดุลลำไส้ กระตุ้นการทำงานของ ระบบภูมิคุ้มกัน ส่งเสริมกระบวนการย่อยอาหาร ลดระดับคอเลสเตอรอล ถึงแม้โพรไบโอติกส์จะมีประโยชน์มากแต่ก็ควรบริโภคในปริมาณที่เหมาะสม หากได้รับมากเกินไปอาจส่งผลเสียต่อร่างกายได้ โดยโพรไบโอติกส์สามารถพบได้ทั้งในแบคทีเรียและยีสต์ที่มีชีวิตหลายชนิด แต่ชนิดที่พบมากที่สุดและถูกใช้ในผลิตภัณฑ์อาหารเสริมมักจะอยู่ในกลุ่มของแบคทีเรียกรดแลคติก
คณะวิทยาศาสตร์
The objective is to develop a web application for tool requests to issues arising from using Excel programs. The initial Excel file is copied from an existing SQL database and repeatedly duplicated, leading to excessive storage consumption. Additionally, the Excel files cannot be accessed concurrently by multiple users. Therefore, this web application aims to connect directly to the SQL database, eliminating the problems caused by using Excel files.
คณะเทคโนโลยีสารสนเทศ
Nowadays, assembling a computer is considered something close to many people. Everyone has a chance to catch it. which knowledge of various components of computers and skills in assembling computers. These 2 things mentioned above are things that the general public should have basic knowledge and understanding about. For the self-assembly of computers, We therefore would like to provide knowledge to the general public who wants to learn how to assemble a computer, including information about its components. Through presentation in the form of learning media using VR technology, which will help reduce the problem of errors. and resources used in assembly Ready to create excitement for users by simulating computer assembly for users to interact within the virtual world. experience and provide knowledge before actually putting it into practice with real equipment This project was therefore created for those interested in assembling computers. Especially for people who have no experience in computer assembly. Including people who would like to have the opportunity to try building a computer by themselves.
คณะเทคโนโลยีการเกษตร
Siamese fighting fish (Betta splendens) is an ornamental fish that is the first exported economically valuable fish in the country, but there is a limitation to increase the production of betta fish due to climate variability and the shortage of Thai workers. This research aims to develop 2 systems: a betta fish fry nursery system and a market-sized betta fish rearing system by using automated technology to precisely control the water quality in the system and reduce labor costs. Using precise automation consists of two systems: a minimal-waste system, which repurposes some of the waste generated from farming, and a zero-waste system, which treats and recycles all wastewater from farming. These systems aim to address issues related to water quality, animal welfare, and labor requirements in Betta fish farming. Experimental results show that these systems improve Betta fish survival rates by 10-15% compared to traditional methods. When considering net returns, the zero- waste system provides the highest profitability.