KMITL Expo 2026 LogoKMITL 66th Anniversary Logo

Power of Leaves: A Natural Fluorescent Seed Coating Innovation for Anti-Counterfeiting

Power of Leaves: A Natural Fluorescent Seed Coating Innovation for Anti-Counterfeiting

Abstract

This experiment aimed to study the suitable types of polymers for coating with chlorophyll extract and the quality of cucumber seeds after coating. The experiment was planned using a Completely Randomized Design (CRD) with four replications, consisting of five methods involving seeds coated with different types of polymers: Polyvinylpyrrolidone, Sodium Alginate, Carboxy Methyl Cellulose, and Hydroxypropyl Methylcellulose, each polymer being coated alongside chlorophyll, with uncoated seeds serving as the control method. The coating substance was prepared by extracting chlorophyll from mango leaves, then mixed with each type of polymer at a concentration of 1%, using an 8% concentration of chlorophyll extract. The properties of each coating method, such as pH and viscosity of the coating substance, were examined before coating the cucumber seeds with a rotary disk coater model RRC150 at a coating rate of 1,100 milliliters per 1 kilogram of seeds. Subsequently, the seeds were dried to reach the initial moisture level using a hot air blower, and seed quality was assessed in various aspects, including seed moisture, germination rate under laboratory conditions, germination index, and seed fluorescence under a portable ultraviolet light illuminator, as well as light emission spectrum analysis using a Spectrophotometer. The experiment found that each type of polymer could be used to form a film together with chlorophyll, which had appropriate pH and viscosity for the coating without affecting seed quality and showed fluorescence on the seed surface both under portable ultraviolet light and spectral emission analysis with a Spectrophotometer. Using HPMC as the film-forming agent with chlorophyll was the most suitable method, enhancing seed fluorescence efficiency.

Objective

สารเรืองแสงที่นิยมใช้เคลือบเมล็ดพันธุ์ในปัจจุบันมีหลายชนิด เช่น Rhodamine B, Curcumin, Auramine O แต่สารเหล่านี้บางชนิดหากใช้ไปเป็นเวลานานอาจเป็นพิษต่อร่างกายมนุษย์ หากสะสมในร่างกายอาจก่อให้เกิด oxidative stress ซึ่งกระตุ้นให้เซลล์เกิดการเสียหาย เพิ่มความเสี่ยงของโรคบางอย่างได้ ในงานวิจัยนี้จึงเลือกใช้สารสกัดจากธรรมชาติมาใช้เคลือบเมล็ดพันธุ์ คือ สารสกัดคลอโรฟิลล์ มีข้อดี คือ หาได้ง่าย มีวิธีการสกัดที่ไม่ซับซ้อน แต่ข้อจำกัดของสารสกัดคลอโรฟิลล์คือมีความสามารถในการเคลือบร่วมกับพอลิเมอร์ได้บางชนิด อาจทำให้ประสิทธิการเรืองแสงมีคุณภาพลดลง ดังนั้นการวิจัยนี้จึงมีวัตถุประสงค์เพื่อหาชนิดของพอลิเมอร์ที่เหมาะสมในการนำมาเคลือบร่วมกับสารสกัดคลอโรฟิลล์แล้วไม่ส่งผลต่อประสิทธิภาพการเรืองแสงและคุณภาพของเมล็ดพันธุ์

Other Innovations

Coffee Nougat Crisp

วิทยาเขตชุมพรเขตรอุดมศักดิ์

Coffee Nougat Crisp

A sweet made from flour and added to give it a chewy texture similar to chewing gum.

Read more
THE DEVELOPMENT OF PACKAGING FOR KLUMKAPIKLONGDANCHUMCHON 3  COMMUNITYENTERPRISE KLONG DAN SUBDISTRICT BANG BO DISTRICT SAMUT PRAKANPROVINCE

คณะเทคโนโลยีการเกษตร

THE DEVELOPMENT OF PACKAGING FOR KLUMKAPIKLONGDANCHUMCHON 3 COMMUNITYENTERPRISE KLONG DAN SUBDISTRICT BANG BO DISTRICT SAMUT PRAKANPROVINCE

This research aimed 1) to study the problems and needs in the packaging design of the community enterprise group 2) to develop packaging for the community enterprise group 3) to study the satisfaction with the packaging design of the members of the Klong Dan Shrimp Paste Community Enterprise Group 3 Klong Dan Subdistrict, Bang Bo District, Samut Prakan Province, consisting of 9 members The study found that the community enterprise group faced the problem of lacking suitable packaging for souvenirs and had a need to develop packaging that is appropriate for this purpose. The packaging material selected was paper, with a rectangular shape including a handle for portability It can be folded for easy transportation and stacked for storage, while maintaining durability The packaging color used was light brown, and the label color was white The label included the following details: shrimp paste recipe, ingredients, manufacturing and expiration dates, the background of the community enterprise group, a QR code, phone number, a short story, group name, production site, as well as illustrations of the group’s location and red krill. The results of developing packaging for the community enterprise group indicated that the new packaging design increased the credibility of the product, building customer confidence in the product. Regarding the satisfaction with the packaging design among the group members, it was found that Packaging Design 1 had the highest level of satisfaction (x ̅ = 4.57, S.D. = 0.22). Among the aspects, color received the highest satisfaction score (x ̅ = 4.74, S.D. = 0.06), followed by the label (x ̅ = 4.69, S.D. = 0.10), while the lowest was the properties aspect, which was rated at a moderate level of satisfaction (x ̅ = 3.83, S.D. = 1.58), respectively.

Read more
The use of Houttuynia cordata Thunb and Piper retrofractum Vahl extracts to inhibit opportunistic infections in patient with atopic dermatitis

คณะวิทยาศาสตร์

The use of Houttuynia cordata Thunb and Piper retrofractum Vahl extracts to inhibit opportunistic infections in patient with atopic dermatitis

Atopic dermatitis patients are the second largest number among skin disease patients. There is no cure for atopic dermatitis, and it can only be treated to relieve symptoms, causing chronic disease. There is a chance that opportunistic infections will enter and cause more disease from the patient's wound, causing the patient to have complications from other infections. This study is interested in studying the reduction of the chance of opportunistic infections in patients with atopic dermatitis using natural extracts. The interest is in Plu Kaow and long pepper because there is data supporting the inhibition of microorganisms. The leaves of both plants are crudely extracted, soaked in 95% ethanol for 7 days, filtered with a Buchner filter, and the extracts are tested for phytochemicals to analyze phenolic components, flavonoids, tannins, anthocyanin, DPPH, and tested for antimicrobial properties. The experiments consisted of 5 types of gram-positive and gram-negative bacteria: E. coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis. The researcher expects that this can be further developed and used to treat patients with atopic dermatitis.

Read more