Mangosteen peel (Garcinia mangostana Linn.) extract using hot water (MPE) has been shown to have antibacterial potential in freshwater sea bass (Lates calcarifer) larvae infected with Aeromonas hydrophila. In vitro studies showed that MPE has a minimum inhibitory concentration (MIC) of 25 ppm and a minimum bactericidal concentration (MBC) of 25 ppm. In vivo, sea bass larvae were immersed in various concentrations of MPE at 0 ppm (control), 20 ppm, 40 ppm and 60 ppm, respectively, for 7 days with A. hydrophila. The results showed that the MPE-treated group had a higher survival rate compared to the control group. Hematological parameters showed that the MPE-treated group had significantly increased red blood cell (RBC), white blood cell (WBC) and hemoglobin (Hb) concentrations compared to the control group. In addition, the water quality parameters were not significantly different, except for ammonia concentration, with MPE having an ammonia concentration of 60 ppm being the lowest. All results can indicate that MPE can improve the antibacterial potential and the culture potential of sea bass larvae.
ปลากะพงขาว (Lates calcarifer) เป็นปลาที่ได้รับความนิยมมากที่สุดในอุตสาหกรรมการเพาะเลี้ยงสัตว์น้ำของไทย โดยเลี้ยงในน้ำจืดและน้ำกร่อย คิดเป็น 95.97% ของพื้นที่เพาะเลี้ยงปลาทะเลทั้งหมด ความสามารถในการปรับตัวและความง่ายในการเลี้ยงในน้ำจืดทำให้ตอบสนองความต้องการได้สูง แต่ยังประสบปัญหาโรคที่เกิดจากแบคทีเรีย เช่น Aeromonas hydrophila ซึ่งก่อให้เกิดโรคร้ายแรงและต้องการวิธีป้องกันที่เป็นมิตรต่อสิ่งแวดล้อม เปลือกมังคุด (Garcinia mangostana Linn.) ที่อุดมไปด้วยสารออกฤทธิ์ทางชีวภาพ แสดงคุณสมบัติต้านแบคทีเรียและเสริมภูมิคุ้มกันในสัตว์น้ำ จากการศึกษาพบว่าเปลือกมังคุดมีประสิทธิภาพในการต้านเชื้อโรคในปลา เช่น Vibrio harveyii และ A. hydrophila ซึ่งเป็นทางเลือกที่ยั่งยืนในการจัดการโรคในฟาร์มปลากะพงขาว
คณะวิทยาศาสตร์
Bacteriocins are microbial peptides that demonstrate potency against pathogens. This study evaluated the inhibitory effects on pathogens and characterized the bacteriogenomic profile of strain TKP1-5, isolated from the feces of Anas platyrhynchos domesticus. Strain TKP1-5 was characterized using phenotypic traits, 16S rRNA sequencing, and Whole-Genome Sequencing (WGS). It exhibited growth in the presence of 2-6% NaCl, temperatures of 25-45°C, and pH levels ranging from 3 to 9. Based on ANIb, ANIm, and dDDH values, strain TKP1-5 was identified as Lactococcus lactis. Whole genome analysis revealed that strain TKP1-5 harbors the Nisin Z peptide gene cluster with a bit-score of 114.775. The antimicrobial spectrum of bacteriocin TKP1-5 showed inhibitory effects against pathogenic bacteria including Pediococcus pentosaceus JCM5885, Listeria monocytogenes ATCC 19115, Enterococcus faecalis JCM 5803T, Salmonella Typhimurium ATCC 13311ᵀ, Aeromonas hydrophila B1 AhB1, Streptococcus agalactiae 1611 and Streptococcus cowan I. Genomic analysis confirmed L. lactis TKP1-5 as a non-human pathogen without antibiotic resistance genes or plasmids. Furthermore, L. lactis TKP1-5 contains potential genes associated with various probiotic properties and health benefits. This suggests that L. lactis TKP1-5, with its antibacterial activity and probiotic potential, could be a promising candidate for further research and application in the food industry.
คณะวิศวกรรมศาสตร์
One of the most important aspects of responding to a medical case is the response time. In general, most fatalities are due to the patient not being able to reach the hands of the doctor in time. This also includes the arrival of medical equipment to the scene. The human brain will start to degrade in function after 3 minutes of oxygen starvation which conventional road transportation method first responders presently use is usually unable to reach the site in this golden 3 minutes, resulting in fatalities during transport or before the arrival of first responders at the scene. Therefore, medical equipment transport by fully autonomous aircraft is explored. This is done through drone deliveries which is much quicker than road methods as the equipment could be flown straight to the site as it is not affected by traffic, road conditions, and navigation. In this project, we will explore an aerial delivery system for medical equipment such as Automatic External Defibrillators (AEDs), First aid equipment, and other small requested medical devices. This will be done through a DJI drone platform and their SDK application. The main goal for this project is to decrease the response time by using an autonomous aerial drone to deliver medical equipment.
คณะครุศาสตร์อุตสาหกรรมและเทคโนโลยี
"Green and Smart City Innovation" is a concrete integration of social innovation and innovation for smart city in Chiang Rai Province with an interdisciplinary collabarative learning approach based on the research and development of learning in the area by the community. Project Title : “APOLE” Cultural Product Design: The Cultural Product Design Beyond. “City development that aims to improve the quality of life By increasing the efficiency of service city management cost reduction and use of resources Emphasis is placed on the participation mechanisms of the public sector, private sector, public sector, and academic sector. Under the concept of developing a livable, modern, sustainable city that provides citizens in the city with a good quality of life. by leveraging technology and innovation as tools” to move towards a Smart City in the future The government sector uses technology as a driving force. Emphasis is placed on creating an infrastructure system. (Infrastructure) to be consistent with the living conditions of local people. By laying down telecommunications infrastructure, smart poles, arranging electrical wires and grounding communication cables. Installation of intelligent CCTV systems, air quality improvement, Internet of Things (IoT) devices, and Internet of Things (IoT) technology control systems, which help improve people's quality of life so that they can live with more quality.