Supplementing broilers with different levels of fructooligosaccharides (FOS) under stress conditions, such as higher stocking densities and recycled litter that were not a significant difference in broiler performance, carcass quality and meat quality between the FOS-supplemented groups and the control group (p>0.05). FOS supplementation improved intestinal health by increasing the villus height to crypt depth ratio Lactobacillus populations increased, and Escherichia coli decreased with FOS supplementation. The heterophil-to-lymphocyte ratio was reduced which indicated lower stress.
การใช้ยาต้านจุลชีพส่งผลเสียเป็นอย่างมากโดยทำให้เชื้อทนทานต่อยาเเละตกค้างในเนื้อสัตว์ได้ ปัจจุบันผู้ส่งออกไก่ใช้น้อยลงหรือไม่ใช้พราะบาง ประเทศห้ามใช้ จึงได้มีการนำสารทีมีความปลอดภัยมาใช้ทดแทนยาต้านจุลชีพ พรีไบโอติก ได้เเก่ FOS เป็นหนึ่งในสารเสริมที่เป็นอาหารของจุลินทรีย์ดีที่มีประโยชน์ในลําไส้ ซึ่งช่วยปรับสภาพเเวดล้อมภายในลำไส้ให้ไก่มีสมรรถนะการเจริญเติบโต สัณฐานวิทยาของลำไส้ ภูมิคุ้ม รวมถึงคุณภาพเนื้อเเละซากให้ดีขึ้นได้"
คณะเทคโนโลยีการเกษตร
This research aimed to (1) analyze the problems and needs in designing labels for catfish strip products of the Lam Sai Phatthana Community Enterprise Group, (2) develop the labels for catfish strip products, and (3) evaluate the satisfaction levels of consumers and community group members with the developed labels. The study involved 17 members of the community enterprise group and 151 consumers. Research methods included in-depth interviews and questionnaires to gather satisfaction data. The results showed that the newly developed product labels were effective in attracting attention and building trust in the product. The satisfaction levels among the Lam Sai Phatthana Community Enterprise Group members and consumers were high (x ̅= 4.17 and 3.75, respectively).
คณะวิทยาศาสตร์
Cancer remains a major global health challenge as the second-leading cause of human death worldwide. The traditional treatments for cancer beyond surgical resection include radiation and chemotherapy; however, these therapies can cause serious adverse side effects due to their high killing potency but low tumor selectivity. The FDA approved monoclonal antibodies (mAbs) that target TIGIT/PVR (T-cell immunoglobulin and ITIM domain/poliovirus receptor) which is an emerging immune checkpoint molecules has been developed; however, the clinical translation of immune checkpoint inhibitors based on antibodies is hampered due to immunogenicity, immunological-related side effects, and high costs, even though these mAbs show promising therapeutic efficacy in clinical trials. To overcome these bottlenecks, small-molecule inhibitors may offer advantages such as better oral bioavailability and tumor penetration compared to mAbs due to their smaller size. Here, we performed structure-based virtual screening of FDA-approved drug repertoires. The 100 screened candidates were further narrowed down to 10 compounds using molecular docking, with binding affinities ranging from -9.152 to -7.643 kcal/mol. These compounds were subsequently evaluated for their pharmacokinetic properties using ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) analysis, which demonstrated favorable drug-like characteristics. The lead compounds will be further analyzed for conformational changes and binding stability against TIGIT through molecular dynamics (MD) simulations to ensure that no significant conformational changes occur in the protein structure. Collectively, this study represents the potential of computational methods and drug repurposing as effective strategies for drug discovery, facilitating the accelerated development of novel cancer treatments.
คณะวิทยาศาสตร์
The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics. Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C. Through comprehensive genomic analysis, strain RRS4 was confirmed to be E. lactis. E. lactis RRS4 demonstrated inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803. Safety assessments via in silico methods, including KEGG annotation, indicated the absence of virulent and undesirable genes in E. lactis RRS4. VirulenceFinder analysis aligned virulence-related genes with those from three strains of E. lactis and four strains of E. faecium. While antibiotic resistance genes were found to be conserved, they did not correlate with key pathogenicity traits. Furthermore, safety evaluations highlighted that E. lactis RRS4 is generally safe, despite the presence of genes associated with antibiotic resistance. Lastly, we propose guidelines for assessing the safety of microbial strains using whole-genome analysis. These findings represent advancements in probiotic research.