A sweet made from flour and added to give it a chewy texture similar to chewing gum.
เพื่อเป็นการฝึกฝนการทำธุรกิจโดยการเริ่มจากการวิเคราะห์และคัดเลือกวัตถุดิบที่เป็นทรัพยากรในพื้นที่และการทำธุรกิจ
คณะแพทยศาสตร์
Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.
คณะบริหารธุรกิจ
Evenly Distributed Temperature Controlled Medical Refrigerator is the medical refrigerator which equipped with specialized control system to control the cold air distribution within the chamber to have the same temperature along the different height within the chamber.
คณะเทคโนโลยีสารสนเทศ
The project of game development to educate about Egyptian civilization This is intended to be a game to promote learning about ancient Egyptian civilization. The original learning may feel boring. Do not attract the attention of learners Therefore saw the presentation in the form of a game on virtual reality technology that inserts knowledge history With the aim of making learning more interesting The organizers can choose to use Unreal Engine 5.1 (Unreal Engine 5.1) and Oculus Quest II (Oculus Quest2) to develop. Within the game, players must find a way to escape from this room within the specified period of solving puzzles in various forms such as statues, traps, etc. In order to solve puzzles and leave this room, players must collect all the information inside the room. In addition, the game shows the life of the player and when the player cannot solve the puzzle correctly.