Since organic rice storage silos were faced with an insect problem, an owner solved this problem using the expert system (ES) in the controlled atmosphere process (CAP) under the required standard, fumigating insects with an N2, reducing O2 concentration to less than 2% for 21 days. This article presents the computational fluid dynamics (CFD) assisted ES successfully solved this problem. First, CFD was employed to determine the gas flow pattern, O2 concentration, proper operating conditions, and a correction factor (K) of silos. As expected, CFD results were consistent with the experimental results and theory, assuring the CFD’s credibility. Significantly, CFD results revealed that the ES controlled N2 distribution throughout the silos and effectively reduced O2 concentration to meet the requirement. Next, the ES was developed based on the inference engine assisted by CFD results and the sweep-through purging principle, and it was implemented in the CAP. Last, the experiments evaluated CAP’s efficacy in controlling O2 concentration and insect extermination in the actual silos. The experimental results and owner’s feedback confirmed the excellent efficacy of ES implementation; therefore, the CAP is effective and practical. The novel aspect of this research is a CFD methodology to create the inference engine and the ES.
การเก็บรักษาข้าวใน ไซโลเก็บข้าวอินทรีย์ เป็นแนวทางสำคัญในการรักษาคุณภาพข้าวและลดการสูญเสียหลังการเก็บเกี่ยว อย่างไรก็ตาม ปัญหาการปนเปื้อนของแมลงศัตรูข้าว เป็นอุปสรรคสำคัญที่ส่งผลกระทบต่อคุณภาพและความปลอดภัยของข้าว โดยทั่วไป การกำจัดแมลงในไซโลมักใช้สารรมยาเคมี เช่น ฟอสฟีน (PH₃) หรือ เมทิลโบรไมด์ (CH₃Br) ซึ่งอาจก่อให้เกิดสารตกค้าง ส่งผลต่อสุขภาพผู้บริโภค และสร้างผลกระทบต่อสิ่งแวดล้อม
คณะวิศวกรรมศาสตร์
The Thai Sign Language Generation System aims to create a comprehensive 3D modeling and animation platform that translates Thai sentences into dynamic and accurate representations of Thai Sign Language (TSL) gestures. This project enhances communication for the Thai deaf community by leveraging a landmark-based approach using a Vector Quantized Variational Autoencoder (VQVAE) and a Large Language Model (LLM) for sign language generation. The system first trains a VQVAE encoder using landmark data extracted from sign videos, allowing it to learn compact latent representations of TSL gestures. These encoded representations are then used to generate additional landmark-based sign sequences, effectively expanding the training dataset using the BigSign ThaiPBS dataset. Once the dataset is augmented, an LLM is trained to output accurate landmark sequences from Thai text inputs, which are then used to animate a 3D model in Blender, ensuring fluid and natural TSL gestures. The project is implemented using Python, incorporating MediaPipe for landmark extraction, OpenCV for real-time image processing, and Blender’s Python API for 3D animation. By integrating AI, VQVAE-based encoding, and LLM-driven landmark generation, this system aspires to bridge the communication gap between written Thai text and expressive TSL gestures, providing the Thai deaf community with an interactive, real-time sign language animation platform.
คณะเทคโนโลยีการเกษตร
Durian is a crucial economic crop of Thailand and one of the most exported agricultural products in the world. However, producing high-quality durian requires maintaining the health of durian trees, ensuring they remain strong and disease-free to optimize productivity and minimize potential damage to both the tree and its fruit. Among the various diseases affecting durian, foliar diseases are among the most common and rapidly spreading, directly impacting tree growth and fruit quality. Therefore, monitoring and controlling leaf diseases is essential for preserving durian quality. This study aims to apply image analysis technology combined with artificial intelligence (AI) to classify diseases in durian leaves, enabling farmers to diagnose diseases independently without relying on experts. The classification includes three categories: healthy leaves (H), leaves infected with anthracnose (A), and leaves affected by algal spot (S). To develop the classification model, convolutional neural network (CNN) algorithms—ResNet-50, GoogleNet, and AlexNet—were employed. Experimental results indicate that the classification accuracy of ResNet-50, GoogleNet, and AlexNet is 93.57%, 93.95%, and 68.69%, respectively.
คณะแพทยศาสตร์
Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.