The study investigated the extraction of astaxanthin-rich oil from shrimp waste biomass, a valuable byproduct rich in functional lipids and proteins. Wet rendering has long been an inexpensive method to extract oil, however the high temperatures and long cooking times negatively affect the amount of astaxanthin. On the other hand, the study looked into employing deep eutectic solvent as a green solvent and combining a wet rendering process with high-shear homogenization and high-frequency ultrasound-assisted extractions. DES-UAE at 60% amplitude and wet rendering at 60 °C were found to be the ideal conditions, as were DES-HAE at 13,000 rpm and wet rendering at 60 °C. With a notable increase in oil yields of 16.80% and 20.12%, respectively, and improved oil quality (lower acid and peroxide values) in comparison to the conventional wet rendering, experimental validation validated the effectiveness of the DES-HAE and DES-UAE procedures. DES-UAE notably raised the amount of astaxanthin. This study demonstrates that DES-HAE and DES-UAE are quicker, lower-temperature substitutes for obtaining premium, astaxanthin-rich shrimp oil, resulting in more effective use of this priceless byproduct.
Sustainability and Waste Utilization: Upcycling shrimp byproducts into valuable oil helps ensure that seafood manufacturing is waste-free. Potential for Nutraceutical & Functional Food Applications
คณะเทคโนโลยีการเกษตร
Threadfin breams is an economically important fish that can be found in fisheries in both the Gulf of Thailand and Andaman Sea and is cheap. In addition, the consumption of raw fish as sashimi is increasingly popular in Thailand. Therefore, it is necessary to promote its consumption to increase its value. This study investigated the preservation of threadfin breams (N. furcosus) for raw or sashimi consumption. The preservation of threadfin breams consisted of Ikejime (K) and cold seawater (S) methods, and the preservation of the fish by Gutting (G) and whole (W) and storing for 3 days on ice (I) or in a refrigerator (F). The freshness quality of threadfin breams was evaluated by sensory, physicochemical (TVB-N, TMA-N and pH), freshness index (Ki-value) and microbiological methods. It was found that after being stored for 3 days, the KGF group of threadfin breams had the highest overall sensory score, which was 8.36±0.80 points, and the KWI, SWI and SWF groups of threadfin breams had the lowest overall sensory scores, which were 8.13±0.77, 8.13±0.77 and 8.13±0.81 points, respectively. And the overall sensory scores of all experimental groups of fish increased significantly (p<0.05). The TVB-N value of threadfin breams in KGF group had the lowest TVB-N value, which was 1.37±0.93 mg nitrogen/100 g sample. The threadfin breams in SGI group had the highest TVB-N value, which was 2.36±1.15 mg nitrogen/100 g sample. The TVB-N of fish in all experimental groups increased significantly (p<0.05). The TMA-N value of threadfin breams in KGF group had the lowest TMA-N value, which was 1.56±0.88 mg nitrogen/100 g sample. The threadfin breams in SWF group had the highest TMA-N value, which was 2.17±1.22 mg nitrogen/100 g sample. The TMA-N of fish in all experimental groups increased significantly (p<0.05). The pH value of threadfin breams in KGF group had the lowest pH value, which was 6.40±0.12. The threadfin breams in SWF group had the highest pH value, which was 6.78±0.25. The pH of fish in all experimental groups increased significantly (p<0.05). Ki value The threadfin breams in KGF group had the lowest Ki value, which was 9.05±0.73%. The threadfin breams in KWI group had the highest Ki value, which was 12.88±4.19%. The Ki value of all experimental groups of fish increased without statistical significance (p>0.05). In terms of freshness quality in microbiology, it was found that in all experimental groups, Salmonella spp., S. aureus, B. cereus, C. perfringens and E. coli were found in all experimental groups of threadfin breams. All types of microorganisms in all groups of threadfin breams increased with statistical significance (p<0.05). When compared with the freshness quality criteria in terms of sensory, chemical, physical, freshness index and microbiology, it was found that all groups of threadfin breams were very fresh and suitable for raw consumption during the preservation and storage for 3 days. After 3 days of storage, the threadfin breams should be consumed cooked because the freshness quality of the fish is not suitable for raw consumption due to the increase in various parameters. The increase in various parameters is due to the deterioration of the fish and the activities of microorganisms. Therefore, threadfin breams is suitable to promote raw consumption within 3 days of storage. In addition, fish preservation, especially by Ikejime method, then cutting open the belly and storing in the refrigerator, can help improve the freshness of the fish. The results of this study can be used to develop techniques for preserving fish after capture for fishermen and can promote the increase in the value of threadfin breams in the future.
คณะเทคโนโลยีการเกษตร
This research aimed 1) to study the problems and needs in the packaging design of the community enterprise group 2) to develop packaging for the community enterprise group 3) to study the satisfaction with the packaging design of the members of the Klong Dan Shrimp Paste Community Enterprise Group 3 Klong Dan Subdistrict, Bang Bo District, Samut Prakan Province, consisting of 9 members The study found that the community enterprise group faced the problem of lacking suitable packaging for souvenirs and had a need to develop packaging that is appropriate for this purpose. The packaging material selected was paper, with a rectangular shape including a handle for portability It can be folded for easy transportation and stacked for storage, while maintaining durability The packaging color used was light brown, and the label color was white The label included the following details: shrimp paste recipe, ingredients, manufacturing and expiration dates, the background of the community enterprise group, a QR code, phone number, a short story, group name, production site, as well as illustrations of the group’s location and red krill. The results of developing packaging for the community enterprise group indicated that the new packaging design increased the credibility of the product, building customer confidence in the product. Regarding the satisfaction with the packaging design among the group members, it was found that Packaging Design 1 had the highest level of satisfaction (x ̅ = 4.57, S.D. = 0.22). Among the aspects, color received the highest satisfaction score (x ̅ = 4.74, S.D. = 0.06), followed by the label (x ̅ = 4.69, S.D. = 0.10), while the lowest was the properties aspect, which was rated at a moderate level of satisfaction (x ̅ = 3.83, S.D. = 1.58), respectively.
คณะวิทยาศาสตร์
Cancer remains a major global health challenge as the second-leading cause of human death worldwide. The traditional treatments for cancer beyond surgical resection include radiation and chemotherapy; however, these therapies can cause serious adverse side effects due to their high killing potency but low tumor selectivity. The FDA approved monoclonal antibodies (mAbs) that target TIGIT/PVR (T-cell immunoglobulin and ITIM domain/poliovirus receptor) which is an emerging immune checkpoint molecules has been developed; however, the clinical translation of immune checkpoint inhibitors based on antibodies is hampered due to immunogenicity, immunological-related side effects, and high costs, even though these mAbs show promising therapeutic efficacy in clinical trials. To overcome these bottlenecks, small-molecule inhibitors may offer advantages such as better oral bioavailability and tumor penetration compared to mAbs due to their smaller size. Here, we performed structure-based virtual screening of FDA-approved drug repertoires. The 100 screened candidates were further narrowed down to 10 compounds using molecular docking, with binding affinities ranging from -9.152 to -7.643 kcal/mol. These compounds were subsequently evaluated for their pharmacokinetic properties using ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) analysis, which demonstrated favorable drug-like characteristics. The lead compounds will be further analyzed for conformational changes and binding stability against TIGIT through molecular dynamics (MD) simulations to ensure that no significant conformational changes occur in the protein structure. Collectively, this study represents the potential of computational methods and drug repurposing as effective strategies for drug discovery, facilitating the accelerated development of novel cancer treatments.