This research aimed to isolate and culture four strains of lactic acid bacteria (LAB) isolated from fermented foods. The antimicrobial activity of the lactic acid bacteria was studied using the agar spot method and the antibiotic resistance properties of the lactic acid bacteria were studied using the agar overlay diffusion method. The results showed that each strain of lactic acid bacteria had different levels of antimicrobial activity and antibiotic resistance, which are safety properties of probiotic microorganisms.
จุลินทรีย์โพรไบโอติก (Probiotic) หมายความว่า จุลินทรีย์ที่มีชีวิต ซึ่งเมื่อร่างกายได้รับในปริมาณที่เพียงพอจะทำให้เกิดผลที่เป็นประโยชน์ต่อสุขภาพ อาหารที่มีการใช้จุลินทรีย์โพรไบโอติกต้องได้รับอนุญาตจากสำนักงานคณะกรรมการ อาหารและยา และ ต้องใช้จุลินทรีย์ตามที่กำหนดไว้ ดังนั้นการวิจัยนี้จึงนำเชื้อแบคทีเรียแลคติกที่แยกได้จากอาหารหมัก 4 สายพันธุ์ มาศึกษาฤทธิ์การต้านเชื้อแบคทีเรียก่อโรคในอาหาร และ ศึกษาสมบัติการดื้อยาของเชื้อแบคทีเรียแลคติก
วิทยาลัยการจัดการนวัตกรรมและอุตสาหกรรม
This research aims to study the waste management process of horse manure, the production process of organic fertilizer from horse waste, and opinions on the use of innovative organic fertilizer from horse manure. A mixed-method approach, combining qualitative and quantitative research, is employed. The organic fertilizer is produced from horse manure, which is a waste that incurs disposal costs. Through the fermentation process, it is transformed into an environmentally friendly fertilizer containing essential nutrients beneficial to plants. According to the laboratory analysis of the organic fertilizer conducted by the Soil Science Laboratory, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, it was found that organic fertilizer from horse manure contains essential nutrients for plant growth, including macronutrients, secondary nutrients, and micronutrients. This reflects the potential of horse waste management, the production process of organic fertilizer from horse manure, the efficiency of the organic fertilizer, and strategies for adding value to expand its commercialization.
คณะวิทยาศาสตร์
Listeriosis is a severe foodborne illness characterized by a fatality rate exceeding 30%, attributed to the pathogen Listeria monocytogenes. This study evaluated 160 lactic acid bacteria (LAB) isolated from Thai pickled crabs for their potential as agents against L. monocytogenes and for their probiotic properties and probiogenomic characteristics. Among these strains, strain DRC3-2 exhibited activity through the synthesis of bacteriocin DRC3-2, which significantly inhibited L. monocytogenes ATCC 19115 in spot-on-lawn assays. Phenotypic and whole-genome analyses revealed that strain DRC3-2 thrived in environments with 2-6% NaCl, pH values ranging from 3 to 9, and temperatures between 25 and 45°C. Based on average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) values, strain DRC3-2 was taxonomically classified as Lactococcus lactis subsp. hordinae. The production of bacteriocin DRC3-2 peaked during the late stationary phase, following its synthesis in the early exponential growth phase. BAGEL4 analysis identified the putative novel bacteriocin DRC3-2 as lactococcin A and B, with respective bit-scores of 40.05 and 36.58. In silico safety assessments confirmed the nonpathogenic nature of strain DRC3-2 in humans, highlighting its absence of antibiotic resistance genes. Finally, this investigation underscores the novel bacteriocin DRC3-2 for application in the prevention and treatment of L. monocytogenes infections.
คณะวิศวกรรมศาสตร์
SecurionSphere is the penetration testing learning platform that focuses on web application exploitation. This platform is intended to address concerns seen in existing penetration testing platforms, such as resource sharing that may affect other users and the constant environment configuration the permits the same response leading to copy the answer from others. Supervisors can use templates to address various forms of web application vulnerability threats. Users can generate the instance of supervisor's templates machine. The platform also randomly generates the environment configuration for each machine has the difference environment and the answer. This allows the users get more realistic learning experiences without affecting the resources of others.