This research aimed to isolate and culture four strains of lactic acid bacteria (LAB) isolated from fermented foods. The antimicrobial activity of the lactic acid bacteria was studied using the agar spot method and the antibiotic resistance properties of the lactic acid bacteria were studied using the agar overlay diffusion method. The results showed that each strain of lactic acid bacteria had different levels of antimicrobial activity and antibiotic resistance, which are safety properties of probiotic microorganisms.
จุลินทรีย์โพรไบโอติก (Probiotic) หมายความว่า จุลินทรีย์ที่มีชีวิต ซึ่งเมื่อร่างกายได้รับในปริมาณที่เพียงพอจะทำให้เกิดผลที่เป็นประโยชน์ต่อสุขภาพ อาหารที่มีการใช้จุลินทรีย์โพรไบโอติกต้องได้รับอนุญาตจากสำนักงานคณะกรรมการ อาหารและยา และ ต้องใช้จุลินทรีย์ตามที่กำหนดไว้ ดังนั้นการวิจัยนี้จึงนำเชื้อแบคทีเรียแลคติกที่แยกได้จากอาหารหมัก 4 สายพันธุ์ มาศึกษาฤทธิ์การต้านเชื้อแบคทีเรียก่อโรคในอาหาร และ ศึกษาสมบัติการดื้อยาของเชื้อแบคทีเรียแลคติก
คณะครุศาสตร์อุตสาหกรรมและเทคโนโลยี
The Department of Engineering Education at KMITL offers courses in power electronics laboratory practices, which require the use of expensive imported training kits. This results in a loss of national revenue due to the purchase of these imported kits. Therefore, the developers propose a power electronics training kit that offers equivalent or superior functionality to the imported ones while being more cost-effective, making it suitable for student experiments.
คณะอุตสาหกรรมอาหาร
This research aims to optimize the production process of gamma-aminobutyric acid (GABA) in fermented pineapple juice using probiotics and acetic acid bacteria (AAB), which are microorganisms with the potential to enhance GABA levels. This process has been developed to improve the nutritional value of fermented pineapple juice and to increase the economic value of Thai pineapples, which have long suffered from low market prices. This study focuses on determining the optimal conditions for GABA production by examining factors such as sugar content, pH levels, fermentation duration, and L-glutamate concentration, as well as the co-cultivation of probiotics and acetic acid bacteria. The experiments are conducted using controlled fermentation techniques, and the bioactive components of the fermented juice are analyzed with advanced instruments such as HPLC and GC-MS. The findings of this research are expected to contribute to the development of formulations and production processes for a high-GABA pineapple-based functional beverage. This product could offer health benefits such as stress reduction, cognitive function enhancement, and relaxation while also strengthening the potential of Thailand’s fermented food and beverage industry.
คณะเทคโนโลยีการเกษตร
Dwarf whipray (Brevitrygon heterura) is a common species found in a local market in the Gulf of Thailand. However, like many other species of stingrays, it is threatened by overfishing and habitat destruction. Therefore, an accurate species identification is crucial because conservation efforts may vary depending on the species. This study aims to understand morphological variation of B. heterura in the Gulf of Thailand by morphometric study and genetic analysis. During October 2022 and February 2023, we obtained 49 samples from research vessels fish landing ports and local fish markets. We observed two distinct groups based on 43 morphological variables/ratios. B. heterura samples from Chanthaburi, Rayong, Chonburi, Samut Sakhon, Nakhon Si Thammarat and Songkla provinces, called “group A," typically have longer snout length than those from Prachuap Khiri Khan provinces, called “group B" according to external morphological characters for species identification. Three morphological variables/ratios were significantly different between groups A and B. Main characters to explain intraspecific variations between group A and group B are further discussed. DNA barcoding based on a fragment of the cytochrome c oxidase subunit I (COI) gene were obtain from eight samples of group A and eight samples from group B. Pairwise percent sequence divergence (p-distance) for COI between group A and group B were 0.0-2.5. This study contributes to the understanding of variations of morphology and genetics of B. heterura in the Gulf of Thailand.