In raising crickets for meat consumption, the growth rate and growth period of crickets are important data used to identify the number of crickets per breeding area at each age. Therefore, the researcher has an idea to create a system for monitoring the growth rate of crickets in a closed system using an infrared camera combined with computer image processing to study the growth and identify the growth period of crickets at each age in order to obtain knowledge that can be disseminated to farmers to improve the breeding process for maximum efficiency.
ปัจจุบันจิ้งหรีดถือได้ว่าเป็นสัตว์เศรษฐกิจชนิดใหม่ของประเทศไทยซึ่งทางภาครัฐโดยเฉพาะกรมปศุสัตว์ได้เริ่มมีการส่งเสริมให้ภาคการเกษตรได้เพาะเลี้ยงจิ้งหรีดเพื่อการบริโภคสำหรับการส่งออก และเป็นการตอบรับกับเทรนด์อุตสาหกรรมอาหารใหม่ (Novel food) ตามแนวทางขององค์การอาหาร และเกษตรแห่งสหประชาชาติ (FAO : Food and Agriculture Organization) ซึ่งคาดการณ์เอาไว้ว่าจำนวนประชากรโลกจะเพิ่มขึ้นอย่างต่อเนื่องทำให้ความต้องการแหล่งโปรตีนมีมากขึ้นตามไปด้วย คณะผู้วิจัยจึงมีแนวความคิดที่จะหาสร้างระบบเลี้ยงจิ้งหรีดที่มีประสิทธิภาพ
คณะเทคโนโลยีการเกษตร
Supplementing broilers with different levels of fructooligosaccharides (FOS) under stress conditions, such as higher stocking densities and recycled litter that were not a significant difference in broiler performance, carcass quality and meat quality between the FOS-supplemented groups and the control group (p>0.05). FOS supplementation improved intestinal health by increasing the villus height to crypt depth ratio Lactobacillus populations increased, and Escherichia coli decreased with FOS supplementation. The heterophil-to-lymphocyte ratio was reduced which indicated lower stress.
คณะทันตแพทยศาสตร์
Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
คณะแพทยศาสตร์
This research studies the effect of CBD on the excitability of mice nociceptive trigeminal ganglion neurons. The electrophysiological parameters of the mouse trigeminal ganglion cells are measured using a Whole Cell Current Clamp Model on a primary cell culture treated with 0.5 uM CBD for 24 hours compared to a control group. After obtaining the results statistical analysis is done using a Mann Whitney-U test.