The designing of mosquitoes counting system instrument is presented in this work. The mosquitoes that were counted died in order not to measure duplicate counting data. As soon as the input source counting machine can detect the mosquito, the single trigger signal is transmitted to the IOT system to interrupt the server immediately. The number of real mosquito is not transmitting to the IOT but only a signal to interrupt the server. The server records the number of the interrupt signal with real-time clock. Then the interrupt information will be further handled. The front end counting machine consist of the high voltage generate with the suitable voltage value and electrode distance for the required mosquitoes size. The low trigger pulse signals of the mosquitoes killed by high voltage are sending to the controller unit. Immediately, interrupt counting signal of the number of mosquitoes is sent to the big stream data collection on IOT system by the time stamp technique. Form the measurement results, 10 live sample mosquitoes in a limited space box to fly though the counting machine show that the count results are 100% correct count.
ประเทศไทยประสบกับปัญหาการแพร่ระบาดของโรคที่มียุงเป็นพาหะนำโรคมานาน เช่น ไข้มาราเลีย โรคไข้เลือดออก โรคเท้าช้าง เป็นต้น โรคไข้เลือดออกถูกพบขึ้นครั้งแรกในประเทศไทยในปี พ.ศ. 2492 ข้อมูลรายงานสถานการณ์โรคไข้เลือดออกตั้งแต่ปี พ.ศ. 2558 ถึงปี พ.ศ. 2563 พบว่ามีผู้ป่วยสูงสุดในปี 2562 ซึ่งพบผู้ป่วยสูงถึง 18,105 รายโดยภาครัฐไม่ได้นิ่งนอนใจเกี่ยวกับปัญหาที่เกิดขึ้นและได้ทำการสนับสนุนนวัตกรรมที่จะเข้ามาช่วยจัดการกับปัญหาดังกล่าว
คณะวิศวกรรมศาสตร์
This study was conducted to develop a prototype cooling cover for transporting raw milk, aiming to provide a solution for maintaining the quality of raw milk during transportation to milk collection centers. The cooling cover is made using Phase Change Material (PCM), produced from water mixed with a gelling agent, in an amount of 5.6 kg, attached around an aluminum milk tank (with a capacity of 25 L). The cover is then covered with a UV-reflective fabric in two types: polyvinyl chloride (PVC) and high-density polyethylene (HDPE). The temperature reduction performance of both types of covers was evaluated by measuring water temperatures at various points along the radial and vertical directions of the milk tank at six points, using type-T thermocouples, under three environmental conditions: a constant temperature of 25 °C, 35 °C, and outdoor ambient temperature (average temperature 35.5 °C) for a minimum duration of 180 min. The experimental results revealed that at 120 min., the water in the tank covered with PCM-PVC and PCM-HDPE covers had temperatures lower than the ambient temperature by 12.6 °C and 12.9 °C, respectively, under a constant ambient temperature of 25 °C, and under a constant ambient temperature of 35 °C lower by 16.7 °C and 16.4 °C, respectively, and outdoor conditions. Since the temperature reduction performance of PCM-PVC and PCM-HDPE covers showed no significant difference, the performance of microbial quality preservation of raw milk was assessed only with PCM-PVC cover in comparison to a non-covered case (control), by measuring coliform and Escherichia coli counts using compact dry plates. Results indicated that after 120 min., milk in the tank covered with PCM-PVC had an average coliform count of 1.6 × 10^4 CFU/ml and E. coli count of 2 × 10^3 CFU/ml, which was lower than the non-covered control with an average coliform count of 1.5 × 10^4 CFU/ml and E. coli count of 1.1 × 10^4 CFU/ml. This study concludes that the temperature reduction achieved by the cooling cover can help inhibit coliform growth to levels below raw milk quality standards, demonstrating the potential of the cooling cover in maintaining the quality and safety of raw milk during transport, ultimately contributing to an improved quality of life for Thai dairy farmers.
คณะวิทยาศาสตร์
Listeriosis is a severe foodborne illness characterized by a fatality rate exceeding 30%, attributed to the pathogen Listeria monocytogenes. This study evaluated 160 lactic acid bacteria (LAB) isolated from Thai pickled crabs for their potential as agents against L. monocytogenes and for their probiotic properties and probiogenomic characteristics. Among these strains, strain DRC3-2 exhibited activity through the synthesis of bacteriocin DRC3-2, which significantly inhibited L. monocytogenes ATCC 19115 in spot-on-lawn assays. Phenotypic and whole-genome analyses revealed that strain DRC3-2 thrived in environments with 2-6% NaCl, pH values ranging from 3 to 9, and temperatures between 25 and 45°C. Based on average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) values, strain DRC3-2 was taxonomically classified as Lactococcus lactis subsp. hordinae. The production of bacteriocin DRC3-2 peaked during the late stationary phase, following its synthesis in the early exponential growth phase. BAGEL4 analysis identified the putative novel bacteriocin DRC3-2 as lactococcin A and B, with respective bit-scores of 40.05 and 36.58. In silico safety assessments confirmed the nonpathogenic nature of strain DRC3-2 in humans, highlighting its absence of antibiotic resistance genes. Finally, this investigation underscores the novel bacteriocin DRC3-2 for application in the prevention and treatment of L. monocytogenes infections.
คณะแพทยศาสตร์
Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.