The designing of mosquitoes counting system instrument is presented in this work. The mosquitoes that were counted died in order not to measure duplicate counting data. As soon as the input source counting machine can detect the mosquito, the single trigger signal is transmitted to the IOT system to interrupt the server immediately. The number of real mosquito is not transmitting to the IOT but only a signal to interrupt the server. The server records the number of the interrupt signal with real-time clock. Then the interrupt information will be further handled. The front end counting machine consist of the high voltage generate with the suitable voltage value and electrode distance for the required mosquitoes size. The low trigger pulse signals of the mosquitoes killed by high voltage are sending to the controller unit. Immediately, interrupt counting signal of the number of mosquitoes is sent to the big stream data collection on IOT system by the time stamp technique. Form the measurement results, 10 live sample mosquitoes in a limited space box to fly though the counting machine show that the count results are 100% correct count.
ประเทศไทยประสบกับปัญหาการแพร่ระบาดของโรคที่มียุงเป็นพาหะนำโรคมานาน เช่น ไข้มาราเลีย โรคไข้เลือดออก โรคเท้าช้าง เป็นต้น โรคไข้เลือดออกถูกพบขึ้นครั้งแรกในประเทศไทยในปี พ.ศ. 2492 ข้อมูลรายงานสถานการณ์โรคไข้เลือดออกตั้งแต่ปี พ.ศ. 2558 ถึงปี พ.ศ. 2563 พบว่ามีผู้ป่วยสูงสุดในปี 2562 ซึ่งพบผู้ป่วยสูงถึง 18,105 รายโดยภาครัฐไม่ได้นิ่งนอนใจเกี่ยวกับปัญหาที่เกิดขึ้นและได้ทำการสนับสนุนนวัตกรรมที่จะเข้ามาช่วยจัดการกับปัญหาดังกล่าว
คณะทันตแพทยศาสตร์
Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
คณะอุตสาหกรรมอาหาร
This research aims to develop plant-based Thai traditional sausage(Sai oua) using young jackfruit as the main raw material. To be an alternative to replace meat by studying the development of formula, changes in physical and physical chemistry during the preservation of the product, young jackfruit has dominant properties of meat-like fibres and can absorb the smell and taste of spices well. The results of the study found that young jackfruit that have been boiled at 100 degrees Celsius for 30 minutes have the fibre closest to cooked chicken. In addition, the study of changes during storage at different temperatures found that the colour and Water Activity (Aw) have changed slightly. While the pH value (pH) decreased and the value of Thiobarbituric Acid Reactive Substances (T-BARS) increased. In terms of texture, it was found that the toughness increased and the elasticity decreased. Compared to the control formula, this study suggests that young jackfruit is a suitable raw material for the production of fillings from plants.
คณะวิศวกรรมศาสตร์
SecurionSphere is the penetration testing learning platform that focuses on web application exploitation. This platform is intended to address concerns seen in existing penetration testing platforms, such as resource sharing that may affect other users and the constant environment configuration the permits the same response leading to copy the answer from others. Supervisors can use templates to address various forms of web application vulnerability threats. Users can generate the instance of supervisor's templates machine. The platform also randomly generates the environment configuration for each machine has the difference environment and the answer. This allows the users get more realistic learning experiences without affecting the resources of others.