Capsicum chinense is a high-potential economic crop in the food and pharmaceutical industries due to its role as a primary source of capsaicin, a bioactive compound with significant physiological effects. However, capsaicin levels and fruit quality are influenced by genetic factors, environmental conditions, and genetic-by-environment (G×E) interactions, leading to variability in capsaicin biosynthesis.

This study aims to:

• Analyze the impact of different environmental conditions on the growth, fruit quality, and capsaicin content of C. chinense ‘Scotch Bonnet’.
• Conduct field experiments at the demonstration plots of the Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, across two growing seasons under four different environmental conditions.
• Establish an F1 hybrid breeding program using six parental lines through a Half-diallel mating design to generate 15 hybrid combinations, assessing General Combining Ability (GCA) and Specific Combining Ability (SCA).

The findings are expected to provide valuable insights into optimizing cultivation conditions for high-pungency chili production and supporting the development of F1 hybrid seeds with commercial viability and consistent capsaicin levels.