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Abstract

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Objective

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Other Innovations

Investigation of Microalgae Chorella sp. KLSc61 crude extract to promote  Lactobacillus plantarum JCM 1149  Growth

คณะวิทยาศาสตร์

Investigation of Microalgae Chorella sp. KLSc61 crude extract to promote Lactobacillus plantarum JCM 1149 Growth

Microalgae are rich in bioactive compounds that may contribute to the growth of probiotics, which require appropriate nutrients, known as prebiotics, to thrive. This study aims to evaluate the effectiveness of crude extracts from intracellular components residues of the microalga Chlorella sp. KLSc61 in promoting the growth of the probiotic bacterium Lactiplantibacillus plantarum JCM1149 under simulated gastrointestinal conditions. The intracellular extracts were obtained using 70% (v/v) ethanol, and their effects on probiotic growth were tested at concentrations of 0.1%, 0.75% and 1.5%. The growth of Lactiplantibacillus plantarum JCM1149 was assessed using the drop plate method. The findings of this study will provide insights into the potential of Chlorella sp. KLSc61 extracts in enhancing probiotic growth, which could lead to the development of synbiotic dietary supplements containing both probiotics and prebiotics. Additionally, this study may serve as a foundation for further research on the role of microalgal extracts in gut health and immune system modulation.

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OPTIMIZATION OF CONCENTRATED BUTTERFLY PEA EXTRACT PROCESS

คณะวิศวกรรมศาสตร์

OPTIMIZATION OF CONCENTRATED BUTTERFLY PEA EXTRACT PROCESS

This thesis project was conducted to identify the optimal conditions for producing concentrated butterfly pea juice using vacuum evaporation to preserve key compounds in butterfly pea flowers, such as anthocyanins—natural pigments with high antioxidant properties. The study applies a Box-Behnken Design, a statistical method that facilitates analysis of multiple factors. The research focuses on the ratio of dried butterfly pea flowers to water, extraction temperature, and evaporation temperature, each of which has a direct effect on the preservation of key compounds, color, aroma, and flavor. The results indicate that using a dried flower-to-water ratio of 1:15, an extraction temperature of 60°C, and an evaporation temperature of 40°C under low pressure can minimize the loss of essential compounds and best retain the properties of the concentrated butterfly pea juice. Findings from this research provide a foundation for developing an industrial production process for concentrated butterfly pea juice and enhance the potential for creating new products from butterfly pea flowers.

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Development of the intelligent indicator label for monitoring rancidity of deep fried foods

คณะวิศวกรรมศาสตร์

Development of the intelligent indicator label for monitoring rancidity of deep fried foods

The production process of the food rancidity indicator label consists of three main steps: 1) preparation of the indicator solution, 2) preparation of the cellulose solution, and 3) formation of the sheet. The indicator solution includes bromothymol blue and methyl red, which act as indicators. The cellulose solution consists of hydroxypropyl methylcellulose, carboxymethyl cellulose, sodium hydroxide, polyethylene glycol 400, and the indicator solution. For the sheet formation, the cellulose solution was mixed with natural latex to increase flexibility and impart hydrophobic properties. After drying, the invention appears as a thin, dark blue label. When exposed to volatile compounds from rancid food, the label changes color from dark blue to green, and then to yellow, corresponding to the increasing amount of volatile compounds from the rancid food.

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