Aggregatibacter actinomycetemcomitans is a key pathogen in periodontal disease, damaging periodontal ligaments and alveolar bone through biofilm formation. D-LL-31, an engineered antimicrobial peptide, exhibits superior biofilm-killing ability compared to conventional treatments, while DNase I enhances its efficacy by disrupting the biofilm matrix. This study evaluated the combined effects of D-LL-31 and DNase I on A. actinomycetemcomitans biofilms. Results showed that D-LL-31 effectively eradicated biofilms, and its combination with DNase I further enhanced biofilm disruption without cytotoxicity to gingival epithelial cells. The D-LL-31 and DNase I combination shows potential for development as a mouthwash to improve oral health and combat periodontal disease.
โรคปริทันต์เป็นปัญหาสุขภาพช่องปากที่พบบ่อย โดยมี Aggregatibacter actinomycetemcomitans (Aa) เป็นหนึ่งในเชื้อก่อโรคสำคัญ เชื้อชนิดนี้สามารถสร้างไบโอฟิล์ม ซึ่งเป็นกลไกหลักที่ช่วยให้เชื้อดื้อยาต้านจุลชีพและหลบเลี่ยงระบบภูมิคุ้มกัน ทำให้การรักษาด้วยยาปฏิชีวนะทั่วไปไม่ได้ผลอย่างมีประสิทธิภาพ การพัฒนาแนวทางใหม่ในการกำจัดไบโอฟิล์มจึงเป็นสิ่งจำเป็น งานวิจัยนี้มุ่งเน้นศึกษาประสิทธิภาพของ D-LL-31 ซึ่งเป็นเปปไทด์ต้านจุลชีพที่ถูกแปลงทางวิศวกรรม เพื่อทำลายเชื้อที่อยู่ในไบโอฟิล์ม และการใช้ DNase I เพื่อสลายโครงสร้างเมทริกซ์ของไบโอฟิล์มร่วมกัน ซึ่งอาจเป็นแนวทางใหม่ในการพัฒนา น้ำยาบ้วนปาก ที่ช่วยลดเชื้อก่อโรคในช่องปากและป้องกันโรคปริทันต์ได้อย่างมีประสิทธิภาพ
คณะวิทยาศาสตร์
Efficient logistics management requires the development of advanced tools to streamline delivery operations. This study aims to optimize vehicle routes for an animal feed store and develop a web-based application for route planning. The research compares two optimization methods: the Branch and Bound Method and Clustering with the Branch and Bound Method. These methods are evaluated against the store’s existing delivery route by analyzing differences in average travel distance across three dependent groups using Repeated Measures ANOVA. The findings reveal a statistically significant difference in average daily travel distances among the three methods at a 0.05 significance level. The Branch and Bound Method yields the shortest average daily travel distance. Additionally, pairwise comparisons of total daily travel distances using the Paired t-test confirm that the Branch and Bound Method produces the most efficient route with statistical significance at 0.05 level. Implementing this method can reduce total travel distance by 957.51, representing a 30.88% reduction, which translates into fuel cost savings of 2,579.45 THB per month. Based on these results, the Branch and Bound Method was selected for implementation in a web-based application. The application features an intuitive user interface, product inventory management, and optimized daily delivery route recommendations for the case study store. Following development, the web application was deployed and tested in real-world operations. The results demonstrate that it effectively provides map-based route recommendations, ensuring ease of use and accessibility on standard mobile devices.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
A Photographic series that expresses the abstract states of myself, towards the question of existence that results from being surrounded by expectations of both surrender and freedom of expression, this series focuses on my own subjectivities in order to bring back memories of almost forgotten feelings and make them clear once more.
คณะอุตสาหกรรมอาหาร
The growing interest in antioxidant-rich foods is driven by their potential to reduce the risk of chronic diseases such as cancer, cardiovascular conditions, and cellular degeneration. Ginger (Zingiber officinale), banana inflorescence (Musa paradisiaca L.), and roselle (Hibiscus sabdariffa L.) are herbal plants known for their high phenolic content, a crucial component in antioxidant activity. However, the bioactive compounds in these plants are often unstable when exposed to light, temperature, and oxygen, leading to a reduction in their efficacy. This study aims to investigate the optimal ratio of ginger, banana inflorescence, and roselle for encapsulation in liposomes—a technique designed to enhance the stability of bioactive compounds and improve their delivery efficacy. The research evaluates the antioxidant activity of the extracts using DPPH, ABTS, and FRAP methods, alongside total phenolic content (TPC) measurement. The most effective ratio for antioxidant activity will be selected for liposomal encapsulation, employing phospholipids as key structural components. The encapsulation efficiency (EE%) will be calculated to assess the effectiveness of the liposomal delivery system. The findings are expected to identify the optimal combination of ginger, banana inflorescence, and roselle that maximizes antioxidant potency and enhances the stability of bioactive compounds through liposomal encapsulation. This approach offers a promising strategy for developing herbal health supplements that maintain their biological properties over time.