Aggregatibacter actinomycetemcomitans is a key pathogen in periodontal disease, damaging periodontal ligaments and alveolar bone through biofilm formation. D-LL-31, an engineered antimicrobial peptide, exhibits superior biofilm-killing ability compared to conventional treatments, while DNase I enhances its efficacy by disrupting the biofilm matrix. This study evaluated the combined effects of D-LL-31 and DNase I on A. actinomycetemcomitans biofilms. Results showed that D-LL-31 effectively eradicated biofilms, and its combination with DNase I further enhanced biofilm disruption without cytotoxicity to gingival epithelial cells. The D-LL-31 and DNase I combination shows potential for development as a mouthwash to improve oral health and combat periodontal disease.
โรคปริทันต์เป็นปัญหาสุขภาพช่องปากที่พบบ่อย โดยมี Aggregatibacter actinomycetemcomitans (Aa) เป็นหนึ่งในเชื้อก่อโรคสำคัญ เชื้อชนิดนี้สามารถสร้างไบโอฟิล์ม ซึ่งเป็นกลไกหลักที่ช่วยให้เชื้อดื้อยาต้านจุลชีพและหลบเลี่ยงระบบภูมิคุ้มกัน ทำให้การรักษาด้วยยาปฏิชีวนะทั่วไปไม่ได้ผลอย่างมีประสิทธิภาพ การพัฒนาแนวทางใหม่ในการกำจัดไบโอฟิล์มจึงเป็นสิ่งจำเป็น งานวิจัยนี้มุ่งเน้นศึกษาประสิทธิภาพของ D-LL-31 ซึ่งเป็นเปปไทด์ต้านจุลชีพที่ถูกแปลงทางวิศวกรรม เพื่อทำลายเชื้อที่อยู่ในไบโอฟิล์ม และการใช้ DNase I เพื่อสลายโครงสร้างเมทริกซ์ของไบโอฟิล์มร่วมกัน ซึ่งอาจเป็นแนวทางใหม่ในการพัฒนา น้ำยาบ้วนปาก ที่ช่วยลดเชื้อก่อโรคในช่องปากและป้องกันโรคปริทันต์ได้อย่างมีประสิทธิภาพ
คณะวิศวกรรมศาสตร์
This project focuses on developing a test device for an AC charger for electric vehicles according to the IEC 61851-1 Annex A standard by simulating the test circuit inside an electric vehicle according to the standard to test the operation of the AC charger. The test topic is related to the communication between the electric vehicle and the charger via a Pulse Width Modulation (PWM) control circuit system and creating an operation manual (WI) to prepare for testing in accordance with ISO/IEC 17025 standards, which are general requirements for laboratory capabilities in conducting tests and/or calibrations. The overall picture of this project is to develop test equipment and create an operation manual by collecting knowledge and various devices and then comparing the data to meet the abovementioned standards to test the Type II AC charger in each state. The test equipment consists of a communication part between the test equipment and the AC charger using a PLC S7-1200 and an HMI to control the operation of the switches in the test equipment circuit, including controlling parameters and displaying results. The equipment used to measure values is an oscilloscope and a multimeter that have undergone a calibration process to comply with the specified standards.
คณะสถาปัตยกรรม ศิลปะและการออกแบบ
The thesis artwork titled “The Red Mist” presents a narrative adapted from a short story of the same name by Assistant Professor Chatnarong Wisutkul in 2003. The story is set in a future world where people's greed and selfishness have led to a war, forcing them to rely on "breathing machines" to survive in the "red toxic mist." Phakin, a 15-year-old boy, embarks on a journey with a group of refugees. As they pass through abandoned cities, they encounter a boy without a breathing machine who has recently lost his father. Phakin decides to help him, despite objections from others. The boy tries to end his life by shutting off his breathing machine, and when Phakin intervenes to save him, he collapses from inhaling the toxic air. Witnessing Phakin's selfless act, the others are moved and join forces to save both of them. Phakin demonstrates that in difficult times, humans must cooperate and help each other rather than being divided and selfish.
คณะอุตสาหกรรมอาหาร
Fish gelatin is increasingly recognized as an alternative source of gelatin, but its use has been limited due to weak gelling properties. To address these issues, the effect of furcellaran, a gelling agent, was examined at various levels (25-100% FG substitution) on the structural and physicochemical properties of FG gels. As the amount of FUR increased to 25%, the FG/FUR gel showed improved hardness and gel strength (P<0.05). Additionally, increasing FUR levels led to higher gelling and melting points, showing a dose-dependent relationship. Microstructural analysis revealed that adding FUR created a denser gel network with smaller gaps. SAXS scattering intensities also increased as FUR concentration rose. Overall, adding FUR improved the gelling properties of FG without negatively affecting springiness and syneresis, enhancing gel strength and gelling temperature.