Aggregatibacter actinomycetemcomitans is a key pathogen in periodontal disease, damaging periodontal ligaments and alveolar bone through biofilm formation. D-LL-31, an engineered antimicrobial peptide, exhibits superior biofilm-killing ability compared to conventional treatments, while DNase I enhances its efficacy by disrupting the biofilm matrix. This study evaluated the combined effects of D-LL-31 and DNase I on A. actinomycetemcomitans biofilms. Results showed that D-LL-31 effectively eradicated biofilms, and its combination with DNase I further enhanced biofilm disruption without cytotoxicity to gingival epithelial cells. The D-LL-31 and DNase I combination shows potential for development as a mouthwash to improve oral health and combat periodontal disease.
โรคปริทันต์เป็นปัญหาสุขภาพช่องปากที่พบบ่อย โดยมี Aggregatibacter actinomycetemcomitans (Aa) เป็นหนึ่งในเชื้อก่อโรคสำคัญ เชื้อชนิดนี้สามารถสร้างไบโอฟิล์ม ซึ่งเป็นกลไกหลักที่ช่วยให้เชื้อดื้อยาต้านจุลชีพและหลบเลี่ยงระบบภูมิคุ้มกัน ทำให้การรักษาด้วยยาปฏิชีวนะทั่วไปไม่ได้ผลอย่างมีประสิทธิภาพ การพัฒนาแนวทางใหม่ในการกำจัดไบโอฟิล์มจึงเป็นสิ่งจำเป็น งานวิจัยนี้มุ่งเน้นศึกษาประสิทธิภาพของ D-LL-31 ซึ่งเป็นเปปไทด์ต้านจุลชีพที่ถูกแปลงทางวิศวกรรม เพื่อทำลายเชื้อที่อยู่ในไบโอฟิล์ม และการใช้ DNase I เพื่อสลายโครงสร้างเมทริกซ์ของไบโอฟิล์มร่วมกัน ซึ่งอาจเป็นแนวทางใหม่ในการพัฒนา น้ำยาบ้วนปาก ที่ช่วยลดเชื้อก่อโรคในช่องปากและป้องกันโรคปริทันต์ได้อย่างมีประสิทธิภาพ
คณะเทคโนโลยีการเกษตร
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คณะเทคโนโลยีการเกษตร
This research gives a comprehensive overview of the use of antibiotics in livestock production, highlighting both the benefits and the risks associated with their use. The benefits, such as improving immunity, digestion, and reducing infections, are contrasted with the growing concern over antibiotic residues and the development of drug resistance. The shift towards alternatives like probiotics is explored as a sustainable solution, with a specific focus on lactic acid bacteria (LAB) found in the digestive systems of livestock. Thailand’s regulations, which control antibiotic use in animal feed, are also discussed, setting the stage for the study on LAB as a potential replacement for imported probiotics. 1. Use of Antibiotics in Livestock: Antibiotics have been used to promote growth, improve digestion, and prevent infections in livestock. However, the improper use of antibiotics can lead to residues in animal products and the development of drug-resistant bacteria. 2. Global Trends in Antibiotic Use: Many countries, like the European Union and Japan, have banned antibiotics as growth promoters, while others, like China and the U.S., are planning similar bans. 3. Thailand's Approach: Thailand has implemented a regulation since September 2020 to control the use of antibiotics in animal feed, requiring control at both feed mills and farms that mix their own feed. 4. Probiotics as an Alternative: Probiotics, particularly lactic acid bacteria (LAB), are being studied as an alternative to antibiotics. LAB are naturally found in the digestive tracts of livestock and are considered beneficial for maintaining gut health and replacing the need for antibiotics. The study examines the potential of LAB from Thai livestock (broilers, pigs, and cattle) as a sustainable alternative to imported probiotics, aiming to overcome issues like low survival rates of foreign probiotics in practice.
คณะวิศวกรรมศาสตร์
During the recent years, PM2.5 concentration is rising above the safety exposure limit in Thailand. PM2.5 could have originated from various sources such as exhaust fumes, open air burning, wildfire, etc. This concludes that all cities or places would have different PM source contributions. Most studies regarding the PM source findings were done based on chemical analysis. Our research team would like to predict the PM sources physically by nanostructures analysis. These methods would require the PM dust to be collected in a limited amount of time and dry. The use of paper filters may cause contamination from filter material which may cause errors in result evaluation. Our team suggests using Electrostatic Precipitator (ESP) where electrostatics is used to capture PM dust. This research mainly focuses on designing and building the ESP system for PM collection whereas the requirement is to collect at least 100 mg of PM dust within 1 day which would be adequate for nanostructure analysis. The study revealed that the customized ESP system could achieve of up to 80% collecting efficiency (which is more than the commercial ESP that we previously used), there’s a also a parametric study of relationships between flow velocity and collecting efficiency where collecting efficiency is inversely proportional to flow velocity. The suggested air velocity is not to exceed 2 m/s. However, there’re still more room for improvement of the ESP system for PM collection such as the convenience of PM collection process which resulted from the ESP construction geometry and sizes.