Coffee is a critical agricultural commodity to be used to produce a premium beverage to serve people worldwide. Coffee microbiome turned to be an essential tool to improve the bean quality through the natural fermentation. Therefore, understanding the microbial diversities could create the final product's better quality. This study investigated the natural microbial consortium during the wet process fermentation of coffee onsite in Thailand to characterize the microorganisms involved in correlation toward the biochemical characteristics and metabolic attributes. Roasting is another important step in developing the complex flavor/ aroma that make coffee to be enjoyable. During the roasting process, the beans undergo many complex and alternatively change in the physicochemical properties from the gained substances in the fermentation process. The changing in the formation of the substances responsible for the sensory qualities, physicochemical/ aroma attributes as well as the health benefits of the final product. Using the starter culture could also develop the distinguished characteristics of coffee (Research collaboration with Van Hart company)
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คณะวิศวกรรมศาสตร์
Jaundice, a common condition in infants that results from high bilirubin levels in the blood, often requires early diagnosis and monitoring to prevent severe complications, especially in newborns. Traditional diagnostic methods can be time-consuming and subject to human error. This study proposes an approach for real-time jaundice detection using advanced image processing techniques and machine learning algorithms. By analyzing images captured in RGB color spaces, pixel values are extracted and processed through Otsu’s thresholding and morphological operations to detect color patterns indicative of jaundice. A classifier model is then trained to distinguish between normal and jaundiced conditions, offering an automated, accurate, and efficient diagnostic tool. The system’s potential to operate in real-time makes it particularly suited for clinical settings, providing healthcare professionals with timely insights to improve patient outcomes. The proposed method represents a significant innovation in healthcare, combining artificial intelligence and medical imaging to enhance the early detection and management of jaundice, reducing reliance on manual interventions and improving overall healthcare delivery.
คณะวิทยาศาสตร์
Bacteriocins are microbial peptides that demonstrate potency against pathogens. This study evaluated the inhibitory effects on pathogens and characterized the bacteriogenomic profile of strain TKP1-5, isolated from the feces of Anas platyrhynchos domesticus. Strain TKP1-5 was characterized using phenotypic traits, 16S rRNA sequencing, and Whole-Genome Sequencing (WGS). It exhibited growth in the presence of 2-6% NaCl, temperatures of 25-45°C, and pH levels ranging from 3 to 9. Based on ANIb, ANIm, and dDDH values, strain TKP1-5 was identified as Lactococcus lactis. Whole genome analysis revealed that strain TKP1-5 harbors the Nisin Z peptide gene cluster with a bit-score of 114.775. The antimicrobial spectrum of bacteriocin TKP1-5 showed inhibitory effects against pathogenic bacteria including Pediococcus pentosaceus JCM5885, Listeria monocytogenes ATCC 19115, Enterococcus faecalis JCM 5803T, Salmonella Typhimurium ATCC 13311ᵀ, Aeromonas hydrophila B1 AhB1, Streptococcus agalactiae 1611 and Streptococcus cowan I. Genomic analysis confirmed L. lactis TKP1-5 as a non-human pathogen without antibiotic resistance genes or plasmids. Furthermore, L. lactis TKP1-5 contains potential genes associated with various probiotic properties and health benefits. This suggests that L. lactis TKP1-5, with its antibacterial activity and probiotic potential, could be a promising candidate for further research and application in the food industry.
วิทยาลัยนวัตกรรมการผลิตขั้นสูง
Since organic rice storage silos were faced with an insect problem, an owner solved this problem using the expert system (ES) in the controlled atmosphere process (CAP) under the required standard, fumigating insects with an N2, reducing O2 concentration to less than 2% for 21 days. This article presents the computational fluid dynamics (CFD) assisted ES successfully solved this problem. First, CFD was employed to determine the gas flow pattern, O2 concentration, proper operating conditions, and a correction factor (K) of silos. As expected, CFD results were consistent with the experimental results and theory, assuring the CFD’s credibility. Significantly, CFD results revealed that the ES controlled N2 distribution throughout the silos and effectively reduced O2 concentration to meet the requirement. Next, the ES was developed based on the inference engine assisted by CFD results and the sweep-through purging principle, and it was implemented in the CAP. Last, the experiments evaluated CAP’s efficacy in controlling O2 concentration and insect extermination in the actual silos. The experimental results and owner’s feedback confirmed the excellent efficacy of ES implementation; therefore, the CAP is effective and practical. The novel aspect of this research is a CFD methodology to create the inference engine and the ES.