Coffee is a critical agricultural commodity to be used to produce a premium beverage to serve people worldwide. Coffee microbiome turned to be an essential tool to improve the bean quality through the natural fermentation. Therefore, understanding the microbial diversities could create the final product's better quality. This study investigated the natural microbial consortium during the wet process fermentation of coffee onsite in Thailand to characterize the microorganisms involved in correlation toward the biochemical characteristics and metabolic attributes. Roasting is another important step in developing the complex flavor/ aroma that make coffee to be enjoyable. During the roasting process, the beans undergo many complex and alternatively change in the physicochemical properties from the gained substances in the fermentation process. The changing in the formation of the substances responsible for the sensory qualities, physicochemical/ aroma attributes as well as the health benefits of the final product. Using the starter culture could also develop the distinguished characteristics of coffee (Research collaboration with Van Hart company)
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วิทยาเขตชุมพรเขตรอุดมศักดิ์
Durian is an important economic crop in Thailand that is affected by foliar diseases such as rust, leaf blight, and leaf spot. These diseases reduce the quality of the yield and increase management costs. This research focuses on developing AI software for screening durian leaf diseases by applying deep learning technology to classify different types of leaf lesions.
คณะวิทยาศาสตร์
With the urgent need for rapid screening of Aflatoxin B1 (AFB1) due to its association with increased liver cirrhosis and hepatocellular carcinoma cases from contaminated agricultural foods, we propose a novel electrochemical aptasensor. This aptasensor is based on trimetallic nanoparticles AuPt-Ru supported by reduced graphene oxide (AuPt-Ru/RGO) modified on a low-cost and disposable goldleaf electrode (GLEAuPt-Ru/RGO) for detection of AFB1. The trimetallic nanoparticle AuPt-Ru was synthesized using an ultrasonic-driven chemical reduction method. The synthesized AuPt-Ru exhibited a waxberry-like appearance, with AuPt core-shell structure and ruthenium dispersed over the particles. The average particle size was 57.35 ± 8.24 nm. The AuPt-Ru was integrated into RGO sheets (inner diameter of 0.5 to 1.6 µm) in order to enhance electron transfer efficiency and increase the specific immobilizing surface area of the thiol-5’-terminated modified aptamer (Apt) to target AFB1. With a large electrochemical surface area and low electrochemical impedance, GLEAuPt-Ru/RGO displays ultra-high sensitivity for AFB1 detection. Differential pulse voltammetry (DPV) measurements revealed a linear range for AFB1 detection range from 0.3 to 30.0 pg mL-1 (R2 = 0.9972), with a limit of detection (LOD, S/N = 3) and a limit of quantification (LOQ, S/N = 10) of 0.009 pg mL-1 and 0.031 pg mL-1, respectively. The developed aptasensor also demonstrated excellent accuracy in real agricultural products, including dried red chili, garlic, peanut, pepper, and Thai jasmine rice, achieving recovery rates between 94.6 and 107.9%. The fabricated aptamer-based GLEAuPt-Ru/RGO performance is comparable to that of a modified commercial electrode, which has great potential application prospects for detecting AFB1 in agricultural products.
คณะอุตสาหกรรมอาหาร
Zero-waste management is crucial for sustainable food systems, promoting the use of agricultural by-products like rice bran. Rich in bioactive polyphenols with antioxidant and antidiabetic properties, rice bran can enhance the nutritional value of food. Polyphenols can slow starch digestion by forming complexes with starch, making them useful for creating low-glycemic foods. While ultrasonication and freeze-thaw treatments have been beneficial individually, their combined effects on starch-polyphenol complexation remain understudied. This study aimed to evaluate the impact of combining these treatments on the interaction between rice starch and red rice bran polyphenols. The dual treatment increased the complexing index, altered functional properties, and affected granule morphology. Structural analysis indicated non-covalent interactions forming non-V-type complexes. Additionally, starch digestibility was reduced, lowering the estimated glycemic index (eGI) compared to the control. These findings suggest a sustainable and green approach to starch modification, with potential for developing functional food products and advancing zero-waste processing.