Expanding from a public park design project to a campus design on an area of over 50 rai in Ang Sila Subdistrict, Mueang District, Chonburi Province, to serve as both an educational institution and a place for relaxation and learning for the surrounding people.
การพัฒนาพื้นที่แห่งนี้ให้กลายเป็นCampus Park เพื่อเป็นพื้นที่พักผ่อนและแลกเปลี่ยนความรู้เกี่ยวกับระบบนิเวศชายหาดของจังหวัดชลบุรี
คณะทันตแพทยศาสตร์
Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.
คณะเทคโนโลยีการเกษตร
Mangosteen peel (Garcinia mangostana Linn.) extract using hot water (MPE) has been shown to have antibacterial potential in freshwater sea bass (Lates calcarifer) larvae infected with Aeromonas hydrophila. In vitro studies showed that MPE has a minimum inhibitory concentration (MIC) of 25 ppm and a minimum bactericidal concentration (MBC) of 25 ppm. In vivo, sea bass larvae were immersed in various concentrations of MPE at 0 ppm (control), 20 ppm, 40 ppm and 60 ppm, respectively, for 7 days with A. hydrophila. The results showed that the MPE-treated group had a higher survival rate compared to the control group. Hematological parameters showed that the MPE-treated group had significantly increased red blood cell (RBC), white blood cell (WBC) and hemoglobin (Hb) concentrations compared to the control group. In addition, the water quality parameters were not significantly different, except for ammonia concentration, with MPE having an ammonia concentration of 60 ppm being the lowest. All results can indicate that MPE can improve the antibacterial potential and the culture potential of sea bass larvae.
คณะเทคโนโลยีการเกษตร
This project involves the development of a plant care system for dormitories using IoT (Internet of Things). The system is implemented through programming on an ESP-32 board and controlled via sensors for automated watering. The commands are operated through smartphones, supporting both iOS and Android. It is expected that this project will make plant care in dormitories easier and more convenient.