Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.
ยีน RGL3 มีบทบาทในการส่งสัญญาณระดับเซลล์ที่สำคัญ และมีความเชื่อมโยงกับความเสี่ยงต่อภาวะความดันโลหิตสูงเนื่องจากการกลายพันธุ์ของยีนในเอ็กซอน 6 การศึกษาความสัมพันธ์เชื่อมโยงในจีโนม (GWAS) แสดงให้เห็นว่า RGL3 เกี่ยวข้องกับภาวะความดันโลหิตสูง ซึ่งทำให้เห็นถึงข้อมูลเชิงลึกเกี่ยวกับพื้นฐานทางพันธุกรรมของภาวะนี้ และอาจช่วยให้ค้นพบผลที่สามารถปกป้องหัวใจและหลอดเลือดจากยีนนี้ แม้ว่าจะมีการค้นพบดังกล่าว แต่ปัจจุบันก็ยังขาดข้อมูลที่ยืนยันบทบาทที่ชัดเจนของ RGL3 ในภาวะความดันโลหิตสูง นอกจากนี้ ผลกระทบทางด้านโครงสร้าง และหน้าที่ของการแปรผันทางพันธุกรรมที่ไม่ทราบนัยยะสำคัญ (VUS) ยังคงไม่มีข้อมูลอธิบายชัดเจน
คณะอุตสาหกรรมอาหาร
This research aims to optimize the production process of gamma-aminobutyric acid (GABA) in fermented pineapple juice using probiotics and acetic acid bacteria (AAB), which are microorganisms with the potential to enhance GABA levels. This process has been developed to improve the nutritional value of fermented pineapple juice and to increase the economic value of Thai pineapples, which have long suffered from low market prices. This study focuses on determining the optimal conditions for GABA production by examining factors such as sugar content, pH levels, fermentation duration, and L-glutamate concentration, as well as the co-cultivation of probiotics and acetic acid bacteria. The experiments are conducted using controlled fermentation techniques, and the bioactive components of the fermented juice are analyzed with advanced instruments such as HPLC and GC-MS. The findings of this research are expected to contribute to the development of formulations and production processes for a high-GABA pineapple-based functional beverage. This product could offer health benefits such as stress reduction, cognitive function enhancement, and relaxation while also strengthening the potential of Thailand’s fermented food and beverage industry.
คณะบริหารธุรกิจ
This research aimed to develop the mixed tea from longan peels and seeds. Population studied were longan farmers who planted longan and preserved the longan product in Ampur Wang Nam Yen, Sa Kaeo Province. From the results, it was found that from By-product in the production of dehydrated longan, longan peels and seeds, which can be processed into ready-to-drink powdered tea. This not only helps reduce waste from the production process but also contributes to generating additional income from these by-products.
คณะเทคโนโลยีการเกษตร
Climate change and the increasing unpredictability of environmental conditions have aggravated the shortage of animal feed crops during the dry season. This study examines effect of packaging thickness on the quality of corn silage during long-term storage, to maintain its nutritional value during feed shortages. The results show that packaging with thicknesses of 80, 120, 150, and 200 microns effectively maintain good physical quality, including odor, texture, color, and pH levels, during the 0–21day storage period. The silage had a fermented like fruit flavor or vinegar flavor, a silage texture, and well-preserved leaves and stems. Its color remained yellowish-green, with pH values between 3.7 and 4.7. Additionally, lactic acid analysis found that silage in 200-micron-thick packaging for 21 days had the highest lactic acid content (5.64%). However, there were no significant differences in the nutritional value of the silage across different packaging thicknesses