Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.
ยีน RGL3 มีบทบาทในการส่งสัญญาณระดับเซลล์ที่สำคัญ และมีความเชื่อมโยงกับความเสี่ยงต่อภาวะความดันโลหิตสูงเนื่องจากการกลายพันธุ์ของยีนในเอ็กซอน 6 การศึกษาความสัมพันธ์เชื่อมโยงในจีโนม (GWAS) แสดงให้เห็นว่า RGL3 เกี่ยวข้องกับภาวะความดันโลหิตสูง ซึ่งทำให้เห็นถึงข้อมูลเชิงลึกเกี่ยวกับพื้นฐานทางพันธุกรรมของภาวะนี้ และอาจช่วยให้ค้นพบผลที่สามารถปกป้องหัวใจและหลอดเลือดจากยีนนี้ แม้ว่าจะมีการค้นพบดังกล่าว แต่ปัจจุบันก็ยังขาดข้อมูลที่ยืนยันบทบาทที่ชัดเจนของ RGL3 ในภาวะความดันโลหิตสูง นอกจากนี้ ผลกระทบทางด้านโครงสร้าง และหน้าที่ของการแปรผันทางพันธุกรรมที่ไม่ทราบนัยยะสำคัญ (VUS) ยังคงไม่มีข้อมูลอธิบายชัดเจน
วิทยาลัยนวัตกรรมการผลิตขั้นสูง
The objective of this research is to utilize waste slag in industrial applications and help mitigate flooding, water accumulation, and ponding issues. Currently, slag from the steel smelting or refining process is commonly used as a component in construction materials, such as road surfaces. However, slag has properties that make it difficult for water to permeate, leading to poor drainage and increased flooding problems. This study focuses on improving the properties of pavement materials to enhance their strength and water permeability. This can be achieved through physical structural modifications or the addition of chemical agents such as HPMC, which increases void spaces to facilitate water absorption and drainage according to required standards. The utilization of waste slag not only helps reduce production costs and improve material performance but also minimizes environmental impacts and promotes the sustainable use of resources.
คณะอุตสาหกรรมอาหาร
The consumption of plant-based products has been gaining popularity as consumers become more health-conscious and aware of environmental impacts. The food industry has been developing meat analogs with properties similar to conventional meat. This study investigates the chemical and physical properties of chickpea-based meat analog burgers and hybrid burgers containing both chickpeas and pork, using the sous-vide cooking method. This technique helps maintain food quality in terms of texture, moisture retention, and nutritional value. The experiment examined various properties of both types of burgers, including cooking loss, water holding capacity, shear force, pH value, and color analysis. Additionally, sensory evaluation was conducted to assess taste, texture, and overall consumer preference. The findings will provide insights into the optimal sous-vide conditions for producing plant-based and hybrid burgers with desirable quality characteristics that meet the needs of health-conscious consumers. This study serves as a valuable guideline for the food industry in developing high-nutritional-value alternative protein products while reducing meat consumption. By incorporating plant-based ingredients, it helps minimize environmental impact and promotes sustainability in food production. The research is significant in both food science and the development of healthier, competitive food products for the future market.
คณะวิทยาศาสตร์
This project aims to investigate and develop an energy storage system utilizing solar energy sources through the integration of solar cell technology and Graphene Quantum Dot Battery, representing a novel approach to enhancing energy storage efficiency and prolonging the lifespan of renewable energy systems. The selection of graphene and quantum dots as materials for battery development is attributed to their exceptional properties, including high electrical conductivity, charge storage capacity, efficient energy transfer, and enhanced stability.