Background: The RGL3 gene plays a role in key signal transduction pathways and has been implicated in hypertension risk through the identification of a copy number variant deletion in exon 6. Genome-wide association studies have highlighted RGL3 as associated with hypertension, providing insights into the genetic underpinnings of the condition and its protective effects on cardiovascular health. Despite these findings, there is a lack of data that confirms the precise role of RGL3 in hypertension. Additionally, the functional impact of certain variants, particularly those classified as variants of uncertain significance, remains poorly understood. Objectives: This study aims to analyze alterations in the RGL3 protein structure caused by mutations and validate the location of the ligand binding sites. Methods: Clinical variants of the RGL3 gene were obtained from NCBI ClinVar. Variants of uncertain significance and likely benign were analyzed. Multiple sequence alignment was conducted using BioEdit v7.7.1. AlphaFold 2 predicted the wild-type and mutant 3D structures, followed by quality assessment via PROCHECK. Functional domain analysis of RasGEF, RASGEF_NTER, and RA domains was performed, and BIOVIA Discovery Studio Visualizer 2024 was used to evaluate structural and physicochemical changes. Results: The analysis of 81 RGL3 variants identified 5 likely benign and 76 variants of uncertain significance (VUS), all of which were missense mutations. Structural modeling using AlphaFold 2 revealed three key domains: RasGEF_NTER, RasGEF, and RA, where mutations induced conformational changes. Ramachandran plot validation confirmed 79.7% of residues in favored regions, indicating an overall reliable structure. Moreover, mutations within RasGEF and RA domains altered polarity, charge, and stability, suggesting potential functional disruptions. These findings provide insight into the structural consequences of RGL3 mutations, contributing to further functional assessments. Discussion & Conclusion: The identified RGL3 mutations induced physicochemical alterations in key domains, affecting charge, polarity, hydrophobicity, and flexibility. These changes likely disrupt interactions with Ras-like GTPases, impairing GDP-GTP exchange and cellular signaling. Structural analysis highlighted mutations in RasGEF and RA domains that may interfere with activation states, potentially affecting protein function and stability. These findings suggest that mutations in RGL3 could have functional consequences, emphasizing the need for further molecular and functional studies to explore their pathogenic potential.
ยีน RGL3 มีบทบาทในการส่งสัญญาณระดับเซลล์ที่สำคัญ และมีความเชื่อมโยงกับความเสี่ยงต่อภาวะความดันโลหิตสูงเนื่องจากการกลายพันธุ์ของยีนในเอ็กซอน 6 การศึกษาความสัมพันธ์เชื่อมโยงในจีโนม (GWAS) แสดงให้เห็นว่า RGL3 เกี่ยวข้องกับภาวะความดันโลหิตสูง ซึ่งทำให้เห็นถึงข้อมูลเชิงลึกเกี่ยวกับพื้นฐานทางพันธุกรรมของภาวะนี้ และอาจช่วยให้ค้นพบผลที่สามารถปกป้องหัวใจและหลอดเลือดจากยีนนี้ แม้ว่าจะมีการค้นพบดังกล่าว แต่ปัจจุบันก็ยังขาดข้อมูลที่ยืนยันบทบาทที่ชัดเจนของ RGL3 ในภาวะความดันโลหิตสูง นอกจากนี้ ผลกระทบทางด้านโครงสร้าง และหน้าที่ของการแปรผันทางพันธุกรรมที่ไม่ทราบนัยยะสำคัญ (VUS) ยังคงไม่มีข้อมูลอธิบายชัดเจน
วิทยาเขตชุมพรเขตรอุดมศักดิ์
This project aims to design and develop an eye-tracking system to facilitate communication for paralyzed immobile patients. The system is designed to enable patients to convey their needs to caregivers or family members by detecting and tracking eye movements using the Tobii Eye Tracker 5 device. This approach serves as an alternative communication method, replacing the physical movement or speech of paralyzed patients. The system effectively detects and tracks eye movements at a distance of 55 to 85 centimeters and is designed for installation on a computer to ensure ease of use. The program interface consists of three main sections: (1) a set of emotions, (2) a set of needs, and (3) a set of additional needs. It supports input from a virtual keyboard in both Thai and English and allows users to specify additional needs through eye-tracking-enabled typing. Furthermore, the system can generate synthetic speech for text that is difficult to pronounce aloud, send notification messages via the Line application, and store usage data in a database presented in a dashboard format. System testing revealed that the optimal detection distance ranges from 65 to 75 centimeters, as this range yields an error rate of no more than 1 percent. The system accurately responds to eye movements for communication through sound within 3 seconds when interacting with various function buttons. This eye-tracking system effectively enables paralyzed immobile patients to communicate their emotions and needs, facilitating better understanding and interaction between patients and their caregivers or family members.
คณะบริหารธุรกิจ
CO Breathalyzer with Voice Response is the device to measured the level of CO residual in a person's lung who consume tobacco. Measuring residual CO in human breath can identify the tobacco addiction level instead of measuring nicotine in blood.
คณะเทคโนโลยีการเกษตร
The design of a 50-rai public park in the Lat Krabang district of Bangkok aims to provide a recreational space for urban residents in Lat Krabang and nearby areas. The focus is on user groups such as students, university students, and working individuals, incorporating the concept of Universal Design to ensure that everyone in society can use the space equally. However, there is still an emphasis on creating active recreational areas to meet the sports and exercise needs of students, university students, and working individuals. The design of the Lat Krabang area, which is a low-lying region resembling a basin, includes features for water retention, water management, and water treatment for use within the park. The area will focus on exercise, sports, running, walking, relaxation, and educational garden spaces.