KMITL Expo 2026 Logo
Half Circle
All Innovation
ป. ตรี โครงงานพิเศษชิ้นงานKMITL Expo 2025Cluster 2025
Bacteriocinogenomic
analysis
and
anti-
pathogenic
activity
of
potential
Lactococcus
lactis
TKP1-
5
isolated
from
the
feces
of
Anas
platyrhynchos
คณะวิทยาศาสตร์, ชีววิทยาประยุกต์, วิทยาศาสตรบัณฑิต สาขาเทคโนโลยีชีวภาพ
AI Translated
Bacteriocinogenomic analysis and anti-pathogenic activity of potential Lactococcus lactis TKP1-5 isolated from the feces of Anas platyrhynchos

Innovation Owner

TP

Mr. THANAPOL PANNGEUN

Student

Details

This study characterizes Lactococcus lactis strain TKP1-5, isolated from duck feces. Genomic analysis identified the Nisin Z peptide gene cluster, and the strain demonstrated significant anti-pathogenic activity and probiotic potential without antibiotic resistance genes.

Bacteriocins are microbial peptides that demonstrate potency against pathogens. This study evaluated the inhibitory effects on pathogens and characterized the bacteriogenomic profile of strain TKP1-5, isolated from the feces of Anas platyrhynchos domesticus.

  • Characterization: Identified as Lactococcus lactis via 16S rRNA sequencing and Whole-Genome Sequencing (WGS) using ANIb, ANIm, and dDDH values.
  • Growth Conditions: Tolerates 2-6% NaCl, temperatures of 25-45°C, and pH levels of 3-9.
  • Bacteriocin Profile: Harbors the Nisin Z peptide gene cluster (bit-score 114.775).
  • Antimicrobial Spectrum: Inhibits pathogens including Pediococcus pentosaceus, Listeria monocytogenes, Enterococcus faecalis, Salmonella Typhimurium, Aeromonas hydrophila, Streptococcus agalactiae, and Streptococcus cowan I.
  • Safety & Potential: Confirmed as a non-human pathogen lacking antibiotic resistance genes or plasmids, while containing genes associated with probiotic properties and health benefits.

Objective

To identify the species of strain TKP1-5 via whole-genome analysis, determine its bacteriocin type, and evaluate its anti-pathogenic activity.

The objectives of this research are:

  1. To study and identify the species of strain TKP1-5 using whole-genome sequencing analysis.
  2. To study and identify the type of bacteriocin produced by strain TKP1-5 through whole-genome analysis.
  3. To study the anti-pathogenic activity of the bacteriocin derived from strain TKP1-5.