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DEVELOPMENT OF CURCUMIN DOUBLE-WALLED BEADS COLORIMETRIC SENSOR FOR DETERMINATION OF PYRIDOXINE (VITAMIN B6) IN DIETARY SUPPLEMENT WITH DETECTION BY IMAGE PROCESSING

Abstract

A smartphone-based colorimetric sensor for quantitative detection of pyridoxine (Vitamin B6, VB-6) in functional drink samples has been realized by developing double layer hydrogel. Electrostatic interaction initiates the cross-linking and produces double layer hydrogel.

Objective

จากสภาพการทำงานและการใช้ชีวิตของมนุษย์ในปัจจุบัน ที่มีความกดดัน เคร่งเครียด ต้องทำงานแข่งกับเวลา ส่งผลให้ผู้คนเริ่มมีปัญหาสุขภาพกันมากขึ้น เนื่องจากขาดการดูแลตัวเองที่ดี การอดอาหาร ตลอดจนการรับประทานอาหารที่ไม่ถูกหลักโภชนาการ เพื่อตอบสนองต่อสังคมที่เร่งรีบ จึงทำให้ในปัจจุบันมีผลิตภัณฑ์เสริมอาหารได้ถูกผลิตขึ้นมาในหลายรูปแบบ ไม่ว่าจะเป็นอาหารเสริมในรูปแบบเม็ด หรือเครื่องดื่มที่มีการเสริมวิตามิน ซึ่งผลิตภัณฑ์เสริมอาหารเหล่านี้ มีจำหน่ายอย่างแพร่หลาย หาซื้อได้ง่ายตามร้านขายยา และร้านสะดวกซื้อต่างๆ จึงทำให้ผู้คนเริ่มให้ความนิยมในการรับประทานผลิตภัณฑ์เสริมอาหารเหล่านี้ หนึ่งในนั้นก็ คือ ผลิตภัณฑ์เสริมอาหารที่มีการเสริมวิตามินบี6 ประเทศไทยในปัจจุบันยังไม่มีข้อกำหนดในการควบคุมคุณภาพผลิตภัณฑ์อาหารเสริม และเครื่องดื่มเสริมวิตามิน ส่งผลให้มีผู้ประกอบการบางรายเติมสารอาหารหรือวิตามินในปริมาณที่ไม่ เป็นไปตามที่ระบุบนฉลาก ดังนั้นจึงมีความจำเป็นในการวิเคราะห์หาสารจำพวกวิตามินในผลิตภัตภัณฑ์เสริมอาหารเพื่อเป็นการควบคุมคุณภาพผลิตภัณฑ์ให้ได้มาตรฐาน และปลอดภัยต่อผู้บริโภค ด้วยเหตุผลข้างต้นนี้ จึงได้มีแนวคิดที่จะพัฒนาวิธีตรวจวัดเชิงสือย่างง่ายในการหาปริมาณวิตามินปี6 ในเครื่องดื่มเสริมวิตามินบี6 ที่สามารถวัดได้อย่างรวดเร็วและแม่นยำ โดยจะทำการสร้างเม็ดบีท ของเคอร์คูมินด้วยวิธี reverse sphenfication เพื่อใช้เป็นตัวรับรู้เชิงสี และใช้ร่วมกับการตรวจวัดด้วยการประมวลผลภาพถ่าย

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