

Innovation Owner
นางสาว Kanjana Sae-Tiew
Advisor
Details
This study examines the effects of chemical mutagens, EMS and colchicine, in inducing mutations in Chrysanthemum through tissue culture to develop novel varieties.
This study examines the effects of chemical mutagens, ethyl methane sulfonate (EMS) and colchicine, in inducing mutations in Chrysanthemum spp. through tissue culture techniques. Key findings include:
- EMS treatment: Significantly increased phenotypic variability, leading to enhanced flower color and size.
- Colchicine treatment: Effectively induced polyploidy, resulting in plants with greater flower size and overall vigor.
Morphological assessments, along with genetic analyses using molecular markers, confirmed the mutations. The integration of chemical mutagenesis with tissue culture presents a promising approach for developing novel Chrysanthemum varieties with improved ornamental traits.

Objective
To improve Chrysanthemum varieties through chemical mutagenesis and tissue culture techniques to develop new, commercially viable ornamental traits.
The objective is to improve Chrysanthemum varieties by:
- Utilizing chemical mutagens (EMS and colchicine) to induce genetic mutations.
- Applying tissue culture techniques to regenerate plants with novel morphological characteristics.
- Developing new, stable varieties suitable for commercial ornamental use.


