Bio-calcium powders were extracted from Asian sea bass bone by heat-treated alkaline with fat removal and bleaching supplementary method. Cereal bars (CBs) were fortified with produced bio-calcium at 3 levels:

• Increased calcium (IS-Ca): calcium ≥10% Thai RDI
• Good source of calcium (GS-Ca): calcium ≥15% Thai RDI
• High calcium (H-Ca): calcium ≥30% Thai RDI

Fortifying with bio-calcium resulted in harder texture CBs. An increase in fortified bio-calcium amounts decreased carbohydrate and fat but increased protein, ash, and calcium content. In conclusion, the production of cereal bars fortified with Asian sea bass bone bio-calcium powder is feasible. Further studies are recommended to analyze calcium bioavailability, sensory acceptance, and shelf life, as well as to check for any remaining hazardous reagents.