This research aims to investigate the adulteration of Khao Dawk Mali 105 rice based on storage age using Near-Infrared Spectroscopy (NIRS) with Fourier Transform Near-Infrared Spectroscopy (FT-NIR) in the wavenumber range of 12,500 – 4,000 cm-1 (800 – 2,500 nm). Storage duration significantly impacts the quality of cooked rice. This research is divided into two parts: 1) to investigate the feasibility of separating rice according to storage age (1, 2, and 3 years) using the best model created by an Ensemble method combined with Second Derivative, which achieved an accuracy of 96.3%. 2) To investigate adulteration based on storage age by adulterating at 0% (all 2- and 3-year-old rice), 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% (all 1-year-old rice). The best model was created using Gaussian Process Regression (GPR) combined with Smoothing + Multiplicative Scatter Correction (MSC), with coefficients of determination (r²), root mean square error of prediction (RMSEP), bias, and prediction ability (RPD) values of 0.92, 8.6%, 0.9%, and 3.6 respectively. This demonstrates that the adulteration model can be applied to separate rice by storage age (1, 2, and 3 years). Additionally, the color values of rice with different storage ages show differences in L* and b* values.
โรงงานผู้ผลิตข้าวพบปัญหาการปลอมปนของข้าวสารที่มีอายุการเก็บรักษาต่างกัน โดยทั่วไปการคัดแยกการปลอมปนจะใช้วิธีมาตรฐานโดยการหุงข้าว จากนั้นนำข้าวหุงสุกไปวัดเนื้อสัมผัสเพื่อแยกอายุของข้าว ซึ่งใช้เวลาและเป็นการทำลายตัวอย่างและเกิดความล่าช้าในการตรวจสอบคุณภาพข้าวสาร งานวิจัยนี้ใช้เทคนิคเนียร์อินฟราเรดสเปกโทรสโกปี (Near-Infrared Spectroscopy, NIRS) ในการตรวจสอบการปลอมปนของข้าวสารพันธุ์ขาวดอกมะลิ 105 (KDML 105) ที่อายุการเก็บรักษาต่างกันเพื่อแก้ไขปัญหาดังกล่าว
คณะวิทยาศาสตร์
Direct Arc Plasma Generator with Six Nozzles, Applications of Plasma Technology and Progress in Nuclear Fusion and Thailand Tokamak-1 (TT1) Development
คณะวิศวกรรมศาสตร์
This thesis presents the development of an IoT system that integrates various communication standards to work together seamlessly. The Core Board serves as the IoT gateway, connecting LoRa, Wi-Fi, and BLE interfaces, and is powered by the STM32H563Zi microcontroller based on the ARM Cortex M33 architecture. The system is designed with slots to support additional communication standards and includes a web platform for data monitoring.
คณะทันตแพทยศาสตร์
Objective or Background: Dental caries is still one of the most significant dental problems worldwide, with prevalence rates up to 90% among children and adults. Cariogenic bacteria, especially Streptococcus mutans, is the primary microorganism involved in the pathogenesis through carbohydrate metabolism and biofilm formation, which are challenging to eradicate. Histatin-5 (HST-5), a human salivary antimicrobial peptide, has demonstrated antimicrobial activity against various fungal and bacterial pathogens. Phytosphingosine (PHS), an endogenous bioactive sphingolipid found in fungi, plants, and humans, also shows antimicrobial properties. This study aimed to evaluate the killing activity of HST-5 alone and in combination with PHS against S. mutans under biofilm-stimulating conditions. Materials and Methods: Antimicrobial activity against a planktonic culture of S. mutans was evaluated using a time-kill assay, and biofilm-forming capacity was confirmed by crystal violet staining assay. The killing ability against 24h pre-formed biofilm was determined using Transferable Solid Phase (TSP) pin lid model. Synergistic activity between HST-5 and PHS was evaluated using the checkerboard technique. Additionally, the cytotoxicity of the tested agent on human gingival fibroblast cells (hGFs) was assessed after 1 h of incubation using an MTT assay. Results: A time-kill assay revealed that both HST-5 and PHS exhibit time- and concentration-dependent activity against the planktonic form of S. mutans. PHS achieved over 90% killing activity within 15 min at 5 μg/ml, whereas HST-5 required 30 min to reach 90% killing at 20 μM. The biofilm formation capacity of S. mutans was confirmed. The inhibitory concentrations (IC50) of HST-5 and PHS against S. mutans biofilm were 25 μM and 13.5 μg/ml, respectively. A synergistic interaction between HST-5 and PHS, with IC50 values reduced by 8-fold and 16-fold, respectively. No cytotoxic effects were observed in hGFs cells at the concentration of the synergistic interaction. Conclusions: Therefore, the combination of HST-5 and PHS may enhance the effectiveness of anti-infective agents against S. mutans biofilm, potentially preventing the development of dental caries.