This research aims to investigate the adulteration of Khao Dawk Mali 105 rice based on storage age using Near-Infrared Spectroscopy (NIRS) with Fourier Transform Near-Infrared Spectroscopy (FT-NIR) in the wavenumber range of 12,500 – 4,000 cm-1 (800 – 2,500 nm). Storage duration significantly impacts the quality of cooked rice. This research is divided into two parts: 1) to investigate the feasibility of separating rice according to storage age (1, 2, and 3 years) using the best model created by an Ensemble method combined with Second Derivative, which achieved an accuracy of 96.3%. 2) To investigate adulteration based on storage age by adulterating at 0% (all 2- and 3-year-old rice), 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100% (all 1-year-old rice). The best model was created using Gaussian Process Regression (GPR) combined with Smoothing + Multiplicative Scatter Correction (MSC), with coefficients of determination (r²), root mean square error of prediction (RMSEP), bias, and prediction ability (RPD) values of 0.92, 8.6%, 0.9%, and 3.6 respectively. This demonstrates that the adulteration model can be applied to separate rice by storage age (1, 2, and 3 years). Additionally, the color values of rice with different storage ages show differences in L* and b* values.
โรงงานผู้ผลิตข้าวพบปัญหาการปลอมปนของข้าวสารที่มีอายุการเก็บรักษาต่างกัน โดยทั่วไปการคัดแยกการปลอมปนจะใช้วิธีมาตรฐานโดยการหุงข้าว จากนั้นนำข้าวหุงสุกไปวัดเนื้อสัมผัสเพื่อแยกอายุของข้าว ซึ่งใช้เวลาและเป็นการทำลายตัวอย่างและเกิดความล่าช้าในการตรวจสอบคุณภาพข้าวสาร งานวิจัยนี้ใช้เทคนิคเนียร์อินฟราเรดสเปกโทรสโกปี (Near-Infrared Spectroscopy, NIRS) ในการตรวจสอบการปลอมปนของข้าวสารพันธุ์ขาวดอกมะลิ 105 (KDML 105) ที่อายุการเก็บรักษาต่างกันเพื่อแก้ไขปัญหาดังกล่าว
คณะวิทยาศาสตร์
Bacteriocins are microbial peptides that demonstrate potency against pathogens. This study evaluated the inhibitory effects on pathogens and characterized the bacteriogenomic profile of strain TKP1-5, isolated from the feces of Anas platyrhynchos domesticus. Strain TKP1-5 was characterized using phenotypic traits, 16S rRNA sequencing, and Whole-Genome Sequencing (WGS). It exhibited growth in the presence of 2-6% NaCl, temperatures of 25-45°C, and pH levels ranging from 3 to 9. Based on ANIb, ANIm, and dDDH values, strain TKP1-5 was identified as Lactococcus lactis. Whole genome analysis revealed that strain TKP1-5 harbors the Nisin Z peptide gene cluster with a bit-score of 114.775. The antimicrobial spectrum of bacteriocin TKP1-5 showed inhibitory effects against pathogenic bacteria including Pediococcus pentosaceus JCM5885, Listeria monocytogenes ATCC 19115, Enterococcus faecalis JCM 5803T, Salmonella Typhimurium ATCC 13311ᵀ, Aeromonas hydrophila B1 AhB1, Streptococcus agalactiae 1611 and Streptococcus cowan I. Genomic analysis confirmed L. lactis TKP1-5 as a non-human pathogen without antibiotic resistance genes or plasmids. Furthermore, L. lactis TKP1-5 contains potential genes associated with various probiotic properties and health benefits. This suggests that L. lactis TKP1-5, with its antibacterial activity and probiotic potential, could be a promising candidate for further research and application in the food industry.
คณะวิทยาศาสตร์
With the urgent need for rapid screening of Aflatoxin B1 (AFB1) due to its association with increased liver cirrhosis and hepatocellular carcinoma cases from contaminated agricultural foods, we propose a novel electrochemical aptasensor. This aptasensor is based on trimetallic nanoparticles AuPt-Ru supported by reduced graphene oxide (AuPt-Ru/RGO) modified on a low-cost and disposable goldleaf electrode (GLEAuPt-Ru/RGO) for detection of AFB1. The trimetallic nanoparticle AuPt-Ru was synthesized using an ultrasonic-driven chemical reduction method. The synthesized AuPt-Ru exhibited a waxberry-like appearance, with AuPt core-shell structure and ruthenium dispersed over the particles. The average particle size was 57.35 ± 8.24 nm. The AuPt-Ru was integrated into RGO sheets (inner diameter of 0.5 to 1.6 µm) in order to enhance electron transfer efficiency and increase the specific immobilizing surface area of the thiol-5’-terminated modified aptamer (Apt) to target AFB1. With a large electrochemical surface area and low electrochemical impedance, GLEAuPt-Ru/RGO displays ultra-high sensitivity for AFB1 detection. Differential pulse voltammetry (DPV) measurements revealed a linear range for AFB1 detection range from 0.3 to 30.0 pg mL-1 (R2 = 0.9972), with a limit of detection (LOD, S/N = 3) and a limit of quantification (LOQ, S/N = 10) of 0.009 pg mL-1 and 0.031 pg mL-1, respectively. The developed aptasensor also demonstrated excellent accuracy in real agricultural products, including dried red chili, garlic, peanut, pepper, and Thai jasmine rice, achieving recovery rates between 94.6 and 107.9%. The fabricated aptamer-based GLEAuPt-Ru/RGO performance is comparable to that of a modified commercial electrode, which has great potential application prospects for detecting AFB1 in agricultural products.
คณะวิทยาศาสตร์
In today’s rapidly expanding e-commerce environment, the massive volume of product reviews makes it crucial to summarize user opinions in a way that is both comprehensible and practically applicable. This research presents a system for analyzing product reviews using Aspect-Based Sentiment Analysis (ABSA), a Natural Language Processing (NLP) technique that identifies key aspects of a review (such as shipping, product quality, and packaging) and evaluates the sentiment (positive, negative, or neutral) associated with each aspect, allowing both consumers and merchants to gain more efficient access to in-depth insights. This project focuses on developing AI for Thai-language ABSA by utilizing WangchanBERTa, a model trained on Thai data, and comparing it with various standard approaches such as TF-IDF + Logistic Regression, Word2Vec + BiLSTM, and Multilingual BERT (mBERT/XLM-R) to assess their performance in terms of accuracy, speed, and resource usage. Additionally, a dashboard visualization is provided to help users quickly grasp review trends. The expected outcome is to create an AI tool that can be practically employed in the e-commerce industry, enabling consumers to make easier purchasing decisions and assisting merchants in effectively improving their products and services.