

Innovation Owner
Mr. NATEE LERDPIRIYASKULKIJ
Student
Details
This research presents a novel colorimetric assay for rapid tannic acid detection in beverages using gallic acid-modified platinum nanoparticles. The sensor utilizes a displacement phenomenon, causing a visible color change from green to orange-brown.
A new colorimetric assay for the rapid detection of tannic acid in beverage samples based on displacement phenomenon of aggregated gallic acid-modified platinum nanoparticles is developed for the first time. PtNPs were functionalized with gallic acid, promoting the formation of the green-hued aggregated nanoparticles. Tannic acid was easily displaced onto the gallic acid-coated PtNPs surfaces, causing dispersion and resulting in a visible color change from green to orange−brown.
- Linear response: 1−2,000 µmol L−1 (R2 = 0.9991)
- LOD: 0.02 µmol L−1
- LOQ: 0.09 µmol L−1
- Precision (RSD): 1.00%−3.36%
- Recoveries: 95.0−104.7%
To address challenges in sensitivity and accuracy caused by anthocyanin interferences, a sample preparation method was developed to degrade anthocyanin in beverages.
Objective
The objectives include optimizing the synthesis of PtNPs for colorimetric sensing, characterizing the particles, developing sample preparation methods for anthocyanin-rich beverages, and establishing a chemical analysis technique for tannic acid detection.
- To study the optimal conditions for the synthesis of platinum nanoparticles (PtNPs) via a simple reduction reaction, using gallic acid as a stabilizing agent for colorimetric sensor applications.
- To characterize the properties of PtNPs using advanced analytical techniques.
- To develop a sample preparation method for beverage samples containing anthocyanin pigments.
- To develop a chemical analysis technique using PtNPs for the detection of tannic acid in beverage samples.


