Innovation Owner
Miss KAEWARIN THAPKLAY
Student
Details
This study isolated Enterococcus lactis RRS4 from Raphanus sativus Linn. and evaluated its probiotic potential and safety. Genomic analysis confirmed its ability to inhibit vancomycin-resistant E. faecalis and established its general safety profile.
The species Enterococcus lactis is closely related to E. faecium and is known for its beneficial and probiotic effects. In this study, strain RRS4 was isolated from Raphanus sativus Linn. and identified based on both phenotypic and genotypic characteristics.
- Viability: Strain RRS4 exhibited cell viability in environments with 2-8% NaCl, pH ranging from 4 to 9, and temperatures between 4°C and 45°C.
- Genomic Analysis: Confirmed RRS4 as E. lactis, demonstrating inhibitory effects against Vancomycin-resistant E. faecalis JCM 5803.
- Safety: In silico assessments indicated the absence of virulent genes. While antibiotic resistance genes were conserved, they did not correlate with key pathogenicity traits.
These findings represent advancements in probiotic research and propose guidelines for assessing the safety of microbial strains using whole-genome analysis.
Objective
The objective is to evaluate the safety and probiotic properties of E. lactis RRS4 isolated from pickled radish. This involves in vitro testing and comparative genomic analysis to ensure its suitability as a future probiotic.
This study aims to evaluate the safety of E. lactis using in vitro hemolysis testing on blood agar, combined with comparative genomic analysis (in silico) using KEGG, CARD, dbCAN, and ResFinder databases to detect antibiotic resistance and virulence-related genes. Additionally, the study investigates the probiotic properties of Enterococcus lactis RRS4, including:
- Phenotypic characteristics
- Tolerance to acid and bile
- Adhesion capabilities
- Antimicrobial activity
These efforts aim to ensure that E. lactis is safe and represents a suitable candidate for future probiotic applications.